ArticleGut pathogens2015
Characterization of the cytolethal distending toxin (typhoid toxin) in non-typhoidal Salmonella serovars.
Article in Gut pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 47 citations in OpenAlex.
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- Functional synergy of heteropentameric B subunits underlies virulence in a Salmonella A2B5 toxin.PLoS pathogens · 2025Article
- The Identification of Enteric Fever-Specific Antigens for Population-Based Serosurveillance.The Journal of infectious diseases · 2024Article
- Typhoid fever.Nature reviews. Disease primers · 2023Review
- The diverse landscape of AB5-type toxins.Engineering microbiology · 2023Review
- Genomic Diversity, Antimicrobial Resistance, Plasmidome, and Virulence Profiles ofAntibiotics (Basel, Switzerland) · 2023Article
- Virulence gene profiles and antimicrobial susceptibility ofJournal of veterinary science · 2023Article
- Human Salmonellosis: A Continuous Global Threat in the Farm-to-Fork Food Safety Continuum.Foods (Basel, Switzerland) · 2023Review
- Genotoxin-producingFrontiers in immunology · 2023Article
- Genomic characterization of invasive typhoidal and non-typhoidal Salmonella in southwestern Nigeria.PLoS neglected tropical diseases · 2022Article
- Whole-Genome Sequencing Provides Insight Into Antimicrobial Resistance and Molecular Characteristics ofFrontiers in microbiology · 2022Article
- The evolutionary diversification of theFrontiers in microbiology · 2022Article
- Antibiotic resistance modifying ability of phytoextracts in anthrax biological agent Bacillus anthracis and emerging superbugs: a review of synergistic mechanisms.Annals of clinical microbiology and antimicrobials · 2021Review
- Diversity and Resistance Profiles of Human Non-typhoidalAntibiotics (Basel, Switzerland) · 2021Article
- Bacterial Toxins Are a Never-Ending Source of Surprises: From Natural Born Killers to Negotiators.Toxins · 2021Review
- The Majority of Typhoid Toxin-PositivemSphere · 2021Article
- Global Genomic Characterization ofFrontiers in microbiology · 2021Article
- Genotoxic Effect ofmBio · 2020Article
- Genomic characterization ofMicrobial genomics · 2020Article
- Bacterial Genotoxin-Induced DNA Damage and Modulation of the Host Immune Microenvironment.Toxins · 2020Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundFor many putative Salmonella enterica subsp. enterica virulence genes, functional characterization across serovars has been limited. Cytolethal distending toxin B (CdtB) is an incompletely characterized virulence factor that is found not only in Salmonella enterica subsp. enterica serovar Typhi (Salmonella Typhi) and dozens of Gram negative bacterial pathogens, but also in non-typhoidal Salmonella (NTS) serovars.
methodsA comparative genomics approach was performed to characterize sequence conservation of the typhoid toxin (TT), encoded in the CdtB-islet, between Salmonella Typhi and NTS serovars. The cytotoxic activity of representative Salmonella enterica subsp. enterica serovars Javiana, Montevideo and Schwarzengrund strains and their respective isogenic cdtB mutants was determined in human intestinal epithelial Henle-407 cells by assessment of cell cycle progression of infected cells using fluorescence-activated cell sorting (FACS). Two-way analysis of variance (ANOVA) was used to determine whether cdtB deletion had a significant (p < 0.05) effect on the percentage of Henle-407 cells at each stage of the cell cycle.
resultsHere we show that a CdtB-islet encoding the cytolethal distending toxin B (CdtB), pertussis-like toxin A (PltA), and pertussis-like toxin B (PltB) is present in a dozen NTS serovars and that these proteins have a high level of sequence conservation and each form monophyletic clades with corresponding Salmonella Typhi genes. Human epithelial Henle-407 cells infected with three representative CdtB-encoding NTS serovars displayed G2/M phase cell cycle arrest that was absent in cells infected with corresponding isogenic cdtB null mutants (p < 0.0001 for the factor ∆cdtB deletion).
conclusionOur results show that CdtB encoded by NTS serovars has a genomic organization, amino acid sequence conservation and biological activity similar to the TT, and thus, may contribute to disease pathogenesis.
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