ArticleGenetics2015
Targeted Gene Disruption by Ectopic Induction of DNA Elimination in Tetrahymena.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Article
- Heterogeneity of radial spoke components in Tetrahymena cilia.Cellular and molecular life sciences : CMLS · 2025Article
- How and when organisms edit their own genomes.Nature genetics · 2025Review
- Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena.The Journal of cell biology · 2025Article
- Close cooperation between Semi1 and Semi2 proteins is essential for pronuclear positioning inMolecular biology of the cell · 2025Article
- Novel requirements for HAP2/GCS1-mediated gamete fusion iniScience · 2024Article
- A family of carboxypeptidases catalyzing α- and β-tubulin tail processing and deglutamylation.Science advances · 2023Article
- TheMolecular biology of the cell · 2023Article
- A practical reference for studying meiosis in the model ciliateMarine life science & technology · 2022Review
- Anterior-posterior pattern formation in ciliates.The Journal of eukaryotic microbiology · 2022Review
- Zfp1, a Cys2His2 zinc finger protein is required for meiosis initiation inCell cycle (Georgetown, Tex.) · 2022Article
- PIWI-Directed DNA Elimination for Tetrahymena Genetics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Arrested crossover precursor structures form stable homologous bonds in a Tetrahymena meiotic mutant.PloS one · 2022Article
- Composition and function of the C1b/C1f region in the ciliary central apparatus.Scientific reports · 2021Article
- Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link.PLoS genetics · 2021Article
- Spatial constraints on chromosomes are instrumental to meiotic pairing.Journal of cell science · 2020Article
- The Transmembrane Protein Semi1 Positions Gamete Nuclei for Reciprocal Fertilization in Tetrahymena.iScience · 2020Article
- Ciliary force-responsive striated fibers promote basal body connections and cortical interactions.The Journal of cell biology · 2020Article
- A chromatin-associated protein required for inducing and limiting meiotic DNA double-strand break formation.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tetrahymena is a useful eukaryotic model for biochemistry and molecular cell biology studies. We previously demonstrated that targeted ectopic DNA elimination, also called co-Deletion (coDel), can be induced by the introduction of an internal eliminated sequence (IES)-target DNA chimeric construct. In this study, we demonstrate that coDel occurs at most of the loci tested and can be used for the production of somatic gene KO strains. We also showed that coDel at two loci can be simultaneously induced by a single transformation; thus, coDel can be used to disrupt multiple gene loci in a single cell. Therefore, coDel is a useful tool for functional genetics in Tetrahymena and further extends the usefulness of this model organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.