Evidence map›Paper›PMID 26205584›Full record

ArticleAnalytical biochemistry2015

A time-resolved Förster resonance energy transfer assay to measure activity of the deamidase of the prokaryotic ubiquitin-like protein.

Ian C Eustis, Jessica Huang, Meagan E Pilkerton, Samuel D Whedon, Champak Chatterjee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ian C EustisDepartment of Chemistry, University of Washington, Seattle, WA 98115, USA.
Jessica HuangDepartment of Chemistry, University of Washington, Seattle, WA 98115, USA.
Meagan E PilkertonDepartment of Chemistry, University of Washington, Seattle, WA 98115, USA.
Samuel D WhedonDepartment of Chemistry, University of Washington, Seattle, WA 98115, USA.
Champak ChatterjeeDepartment of Chemistry, University of Washington, Seattle, WA 98115, USA. Electronic address: chatterjee@chem.washington.edu.
University of Washington · US

Funding

Chemical Strategies to Investigate Gene Regulation By Histone SumoylationR01GM110430 · NIGMS · UNIVERSITY OF WASHINGTON · PI CHATTERJEE, CHAMPAK · 2014 to 2022
$2.5M
NIGMS NIH HHS GM110430-01NIGMS NIH HHS R01 GM110430
6 · The paper itself

Abstract

The modification of proteins in Mycobacterium tuberculosis (Mtb) by the prokaryotic ubiquitin-like protein (Pup) targets them for degradation by mycobacterial proteasomes. Although functionally similar to eukaryotic deubiquitylating enzymes, the deamidase of Pup, called Dop, has no known mammalian homologs. Because Dop is necessary for persistent infection by Mtb, its selective inhibition holds potential for tuberculosis therapy. To facilitate high-throughput screens for Dop inhibitors, we developed a time-resolved Förster resonance energy transfer (TR-FRET)-based assay for Dop function. The TR-FRET assay was successfully applied to determine the Michaelis constant for adenosine triphosphate (ATP) binding and to test the cofactor tolerance of Dop.

Indexed as

Fluorescence Resonance Energy TransferAdenosine TriphosphateBacterial ProteinsMycobacterium tuberculosisProteasome Endopeptidase ComplexTime FactorsAdenosine TriphosphateBacterial ProteinsProteasome Endopeptidase ComplexDeamidase of PupLigaseProkaryotic ubiquitin-like proteinPupylationTR–FRETTuberculosis

Identifiers

PMID26205584
PMCPMC4560620
OpenAlexW1159624361

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.