ArticleIntegrative biology : quantitative biosciences from nano to macro2015
Cell spreading area regulates clathrin-coated pit dynamics on micropatterned substrate.
Article in Integrative biology : quantitative biosciences from nano to macro, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Unjamming Transition as a Paradigm for Biomechanical Control of Cancer Metastasis.Cytoskeleton (Hoboken, N.J.) · 2025Review
- The Plasma Membrane and Mechanoregulation in Cells.ACS omega · 2024Review
- Cellular mechanotransduction of human osteoblasts in microgravity.NPJ microgravity · 2024Article
- Cellular mechanotransduction of human osteoblasts in microgravity.bioRxiv : the preprint server for biology · 2024Article
- Substrate stiffness reduces particle uptake by epithelial cells and macrophages in a size-dependent manner through mechanoregulation.Nanoscale · 2022Article
- Compressive stress drives adhesion-dependent unjamming transitions in breast cancer cell migration.Frontiers in cell and developmental biology · 2022Article
- Dynamic interplay between cell membrane tension and clathrin-mediated endocytosis.Biology of the cell · 2021Review
- A high-resolution real-time quantification of astrocyte cytokine secretion under shear stress for investigating hydrocephalus shunt failure.Communications biology · 2021Article
- Complimentary action of structured and unstructured domains of epsin supports clathrin-mediated endocytosis at high tension.Communications biology · 2020Article
- Ultrastructural Analysis of Vesicular Transport in Electrotransfection.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2018Article
- Article
- Loss of PTEN promotes formation of signaling-capable clathrin-coated pits.Journal of cell science · 2018Article
- Controlling Cellular Volume via Mechanical and Physical Properties of Substrate.Biophysical journal · 2018Article
- A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns.Frontiers in chemistry · 2018Article
- Membrane Tension Inhibits Rapid and Slow Endocytosis in Secretory Cells.Biophysical journal · 2017Article
- New advances in probing cell-extracellular matrix interactions.Integrative biology : quantitative biosciences from nano to macro · 2017Review
- A Microfabricated 96-Well 3D Assay Enabling High-Throughput Quantification of Cellular Invasion Capabilities.Scientific reports · 2017Article
- Clathrin polymerization exhibits high mechano-geometric sensitivity.Soft matter · 2017Article
- Biophysical Tools for Cellular and Subcellular Mechanical Actuation of Cell Signaling.Biophysical journal · 2016Review
- Development of an advanced microfluidic micropipette aspiration device for single cell mechanics studies.Biomicrofluidics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Clathrin-mediated endocytosis (CME) is the most characterized pathway for the endocytic entry of proteins and lipids at the plasma membrane of eukaryotic cells. Numerous studies have probed the roles of different endocytic accessory proteins in regulating the dynamics of clathrin-coated pit (CCP) assembly. However, it is not completely clear how physical cues regulate CCP dynamics. Here we employ microcontact printing to control cell shape and examine CCP dynamics as a function of cell spreading area for three differently sized cells. Cells with a large spreading area had more short-lived CCPs but a higher CCP initiation rate. Interestingly, we found that fluorescence intensity of CCPs decreased with increasing cell spreading area in a manner that was dependent on the cortical actin network. Our results point to another facet of the regulation of CCP dynamics, suggesting that CME may be modulated while cells change their mechanical state and remodel their actin cytoskeleton during various processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.