ArticleScientific reports2015
A large-scale in vivo RNAi screen to identify genes involved in Notch-mediated follicle cell differentiation and cell cycle switches.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Identification and expression analysis of heat shock protein family genes of gall fly (Procecidochares utilis) under temperature stress.Cell stress & chaperones · 2023Article
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- Reproduction Evaluation and Transcription Analysis ofInsects · 2022Article
- Drosophila FMRP controls miR-276-mediated regulation of nejire mRNA for space-filling dendrite development.G3 (Bethesda, Md.) · 2022Article
- Characterization of reproductive proteins in the Mexican fruit fly points towards the evolution of novel functions.Proceedings. Biological sciences · 2022Article
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- Analysis of the Temporal Patterning of Notch Downstream Targets during Drosophila melanogaster Egg Chamber Development.Scientific reports · 2020Article
- Downregulation of homeodomain protein Cut is essential forDevelopment (Cambridge, England) · 2019Article
- Functional Interactions BetweenG3 (Bethesda, Md.) · 2018Article
- Article
- Integration of Drosophila and Human Genetics to Understand Notch Signaling Related Diseases.Advances in experimental medicine and biology · 2018Review
- AMP-activated protein kinase has diet-dependent and -independent roles in Drosophila oogenesis.Developmental biology · 2016Article
- A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis.G3 (Bethesda, Md.) · 2016Article
- Automatic stage identification of Drosophila egg chamber based on DAPI images.Scientific reports · 2016Article
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Authors and funding
8 authors.
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Abstract
During Drosophila oogenesis, follicle cells sequentially undergo three distinct cell-cycle programs: the mitotic cycle, endocycle, and gene amplification. Notch signaling plays a central role in regulating follicle-cell differentiation and cell-cycle switches; its activation is essential for the mitotic cycle/endocycle (M/E) switch. Cut, a linker between Notch signaling and cell-cycle regulators, is specifically downregulated by Notch during the endocycle stage. To determine how signaling pathways coordinate during the M/E switch and to identify novel genes involved in follicle cell differentiation, we performed an in vivo RNAi screen through induced knockdown of gene expression and examination of Cut expression in follicle cells. We screened 2205 RNAi lines and found 33 genes regulating Cut expression during the M/E switch. These genes were confirmed with the staining of two other Notch signaling downstream factors, Hindsight and Broad, and validated with multiple independent RNAi lines. We applied gene ontology software to find enriched biological meaning and compared our results with other publications to find conserved genes across tissues. Specifically, we found earlier endocycle entry in anterior follicle cells than those in the posterior, identified that the insulin-PI3K pathway participates in the precise M/E switch, and suggested Nejire as a cofactor of Notch signaling during oogenesis.
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