ArticleJournal of the American Society for Mass Spectrometry2015
Ion Mobility Mass Spectrometry for Ion Recovery and Clean-Up of MS and MS/MS Spectra Obtained from Low Abundance Viral Samples.
Article in Journal of the American Society for Mass Spectrometry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Gas Phase Separation of Modified Peptides for Activity-Based Protein Profiling.Analytical chemistry · 2025Article
- Critical review on in silico methods for structural annotation of chemicals detected with LC/HRMS non-targeted screening.Analytical and bioanalytical chemistry · 2025Review
- Ion Mobility Mass Spectrometry (IM-MS) for Structural Biology: Insights Gained by Measuring Mass, Charge, and Collision Cross Section.Chemical reviews · 2023Review
- Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.Chemical reviews · 2022Review
- Utilizing liquid chromatography, ion mobility spectrometry, and mass spectrometry to assess INLIGHT™ derivatized N-linked glycans in biological samples.Analytical and bioanalytical chemistry · 2022Article
- Identification of N-glycans with GalNAc-containing antennae from recombinant HIV trimers by ion mobility and negative ion fragmentation.Analytical and bioanalytical chemistry · 2021Article
- Identification of Isomeric N-Glycans by Conformer Distribution Fingerprinting using Ion Mobility Mass Spectrometry.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- The expanding role of mass spectrometry in the field of vaccine development.Mass spectrometry reviews · 2020Review
- Applications of Ion Mobility-Mass Spectrometry in Carbohydrate Chemistry and Glycobiology.Molecules (Basel, Switzerland) · 2018Review
- Signature of Antibody Domain Exchange by Native Mass Spectrometry and Collision-Induced Unfolding.Analytical chemistry · 2018Article
- Structural Studies of Fucosylated N-Glycans by Ion Mobility Mass Spectrometry and Collision-Induced Fragmentation of Negative Ions.Journal of the American Society for Mass Spectrometry · 2018Article
- Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans.Journal of the American Society for Mass Spectrometry · 2018Article
- Isomer Information from Ion Mobility Separation of High-Mannose Glycan Fragments.Journal of the American Society for Mass Spectrometry · 2018Article
- Recent advances in ion mobility-mass spectrometry for improved structural characterization of glycans and glycoconjugates.Current opinion in chemical biology · 2018Review
- Glycosylation profiling to evaluate glycoprotein immunogens against HIV-1.Expert review of proteomics · 2017Review
- Structural principles controlling HIV envelope glycosylation.Current opinion in structural biology · 2017Review
- Travelling-wave ion mobility mass spectrometry and negative ion fragmentation of hybrid and complex N-glycans.Journal of mass spectrometry : JMS · 2016Article
- Determination of ion mobility collision cross sections for unresolved isomeric mixtures using tandem mass spectrometry and chemometric deconvolution.Analytica chimica acta · 2016Article
- Advanced Multidimensional Separations in Mass Spectrometry: Navigating the Big Data Deluge.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Article
- Travelling-wave ion mobility and negative ion fragmentation of high-mannose N-glycans.Journal of mass spectrometry : JMS · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Many samples of complex mixtures of N-glycans released from small amounts of material, such as glycoproteins from viruses, present problems for mass spectrometric analysis because of the presence of contaminating material that is difficult to remove by conventional methods without involving sample loss. This study describes the use of ion mobility for extraction of glycan profiles from such samples and for obtaining clean CID spectra when targeted m/z values capture additional ions from those of the target compound. N-glycans were released enzymatically from within SDS-PAGE gels, from the representative recombinant glycoprotein, gp120 of the human immunodeficiency virus, and examined by direct infusion electrospray in negative mode followed by ion mobility with a Waters Synapt G2 mass spectrometer (Waters MS-Technologies, Manchester, UK). Clean profiles of singly, doubly, and triply charged N-glycans were obtained from samples in cases where the raw electrospray spectra displayed only a few glycan ions as the result of low sample concentration or the presence of contamination. Ion mobility also enabled uncontaminated CID spectra to be obtained from glycans when their molecular ions displayed coincidence with ions from fragments or multiply charged ions with similar m/z values. This technique proved to be invaluable for removing extraneous ions from many CID spectra. The presence of such ions often produces spectra that are difficult to interpret. Most CID spectra, even those from abundant glycan constituents, benefited from such clean-up, showing that the extra dimension provided by ion mobility was invaluable for studies of this type.
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