Evidence map›Paper›PMID 26204518›Full record

ArticlePloS one2015

De Novo Assembly and Annotation of the Chinese Chive (Allium tuberosum Rottler ex Spr.) Transcriptome Using the Illumina Platform.

Shu-Mei Zhou, Li-Mei Chen, Shi-Qi Liu, Xiu-Feng Wang, Xiu-Dong Sun

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
7.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
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  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Transcriptome analysis and genetic diversity ofMitochondrial DNA. Part B, Resources · 2021
    Article
  11. Article
  12. Omics approaches inPeerJ · 2020
    Article
  13. Article
  14. Article
  15. Therapeutic Role of Functional Components in Alliums for Preventive Chronic Disease in Human Being.Evidence-based complementary and alternative medicine : eCAM · 2017
    Review
  16. The Amaryllidaceae alkaloids: biosynthesis and methods for enzyme discovery.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2016
    Article
  17. Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Shu-Mei ZhouState Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
Li-Mei ChenState Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
Shi-Qi LiuState Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
Xiu-Feng WangState Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
Xiu-Dong SunState Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
Shandong Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chinese chive (A. tuberosum Rottler ex Spr.) is one of the most widely cultivated Allium species in China. However, minimal transcriptomic and genomic data are available to reveal its evolution and genetic diversity. In this study, de novo transcriptome sequencing was performed to produce large transcript sequences using an Illumina HiSeq 2000 instrument. We produced 51,968,882 high-quality clean reads and assembled them into 150,154 contigs. A total of 60,031 unigenes with an average length of 631 bp were identified. Of these, 36,523 unigenes were homologous to existing database sequences, 35,648 unigenes were annotated in the NCBI non-redundant (Nr) sequence database, and 23,509 unigenes were annotated in the Swiss-Prot database. A total of 26,798 unigenes were assigned to 57 Gene Ontology (GO) terms, and 13,378 unigenes were assigned to Cluster of Orthologous Group categories. Using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, we mapped 21,361 unigenes onto 128 pathways. Furthermore, 2,125 sequences containing simple sequence repeats (SSRs) were identified. This new dataset provides the most comprehensive resource currently available for gene expression, gene discovery, and future genomic research on Chinese chive. The sequence resources developed in this study can be used to develop molecular markers that will facilitate further genetic research on Chinese chive and related species.

Indexed as

Genes, PlantTranscriptomeChiveDatabases, ProteinGenetic VariationHigh-Throughput Nucleotide SequencingMolecular Sequence Annotation

Identifiers

PMID26204518
PMCPMC4512717
OpenAlexW962092474

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.