ArticleNature protocols2015
Superresolution live imaging of plant cells using structured illumination microscopy.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 70 citations in OpenAlex.
- Mechanical constraint causes lower turgor, thicker walls, and faster growth in Arabidopsis root hairs.Plant physiology · 2026Article
- Root expansion microscopy: A robust method for super resolution imaging in Arabidopsis.The Plant cell · 2025Article
- Variable-Angle Epifluorescence Microscopy for Single-Particle Tracking in the Plant ER.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes.Chromosoma · 2023Article
- Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology.Plant biotechnology journal · 2023Review
- Fluorescent Fusion Protein Expression in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Fluorescent Nanoparticles for Super-Resolution Imaging.Chemical reviews · 2022Review
- Correlation of vacuole morphology with stomatal lineage development by whole-cell electron tomography.Plant physiology · 2022Article
- Super-resolution imaging of Douglas fir xylem cell wall nanostructure using SRRF microscopy.Plant methods · 2022Article
- Imaging the living plant cell: From probes to quantification.The Plant cell · 2022Review
- Super-resolution imaging illuminates new dynamic behaviors of cellulose synthase.The Plant cell · 2022Article
- Imaging tools for plant nanobiotechnology.Frontiers in genome editing · 2022Review
- Organization of peptidoglycan synthesis in nodes and separate rings at different stages of cell division of Streptococcus pneumoniae.Molecular microbiology · 2021Article
- GR24, A Synthetic Strigolactone Analog, and Light Affect the Organization of Cortical Microtubules in Arabidopsis Hypocotyl Cells.Frontiers in plant science · 2021Article
- Concepts and Application of DNA Origami and DNA Self-Assembly: A Systematic Review.Applied bionics and biomechanics · 2021Review
- From Microscopy to Nanoscopy: Defining anFrontiers in plant science · 2021Review
- Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum.Nature communications · 2020Article
- Imaging flowers: a guide to current microscopy and tomography techniques to study flower development.Journal of experimental botany · 2020Review
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Arabinogalactan protein-rare earth element complexes activate plant endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although superresolution (SR) approaches have been routinely used for fixed or living material from other organisms, the use of time-lapse structured illumination microscopy (SIM) imaging in plant cells still remains under-developed. Here we describe a validated method for time-lapse SIM that focuses on cortical microtubules of different plant cell types. By using one of the existing commercially available SIM platforms, we provide a user-friendly and easy-to-follow protocol that may be widely applied to the imaging of plant cells. This protocol includes steps describing calibration of the microscope and channel alignment, generation of an experimental point spread function (PSF), preparation of appropriate observation chambers with available plant material, image acquisition, reconstruction and validation. This protocol can be carried out within two to three working days.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.