ArticleBiomedical optics express2015
Photoacoustic imaging of fluorophores using pump-probe excitation.
Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Probing the photophysical properties of fluorescent proteins using photoacoustic pump-probe spectroscopy and imaging.Photoacoustics · 2025Article
- Characterizing a photoacoustic and fluorescence imaging platform for preclinical murine longitudinal studies.Journal of biomedical optics · 2023Article
- Arylphosphonate-Tethered Porphyrins: Fluorescence Silencing Speaks a Metal Language in Living Enterocytes*.Chembiochem : a European journal of chemical biology · 2021Article
- Synchronously Amplified Photoacoustic Image Recovery (SAPhIRe).Photoacoustics · 2020Article
- Spontaneous loss versus stimulation gain in pump-probe microscopy: a proof of concept demonstration.Journal of biomedical optics · 2020Article
- Bioengineered bacterial vesicles as biological nano-heaters for optoacoustic imaging.Nature communications · 2019Article
- Technology Advancements in Blood Coagulation Measurements for Point-of-Care Diagnostic Testing.Frontiers in bioengineering and biotechnology · 2019Review
- Advanced optoacoustic methods for multiscale imaging of in vivo dynamics.Chemical Society reviews · 2017Review
- Photoacoustic pump-probe tomography of fluorophores in vivo using interleaved image acquisition for motion suppression.Scientific reports · 2017Article
- Emerging concepts in functional and molecular photoacoustic imaging.Current opinion in chemical biology · 2016Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A pump-probe technique for the detection of fluorophores in tomographic PA images is introduced. It is based on inducing stimulated emission in fluorescent molecules, which in turn modulates the amount of thermalized energy, and hence the PA signal amplitude. A theoretical model of the PA signal generation in fluorophores is presented and experimentally validated on cuvette measurements made in solutions of Rhodamine 6G, a fluorophore of known optical and molecular properties. The application of this technique to deep tissue tomographic PA imaging is demonstrated by determining the spatial distribution of a near-infrared fluorophore in a tissue phantom.
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Registered trials
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