ArticleStandards in genomic sciences2015
Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat.
Article in Standards in genomic sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- The unique and enigmatic spirochete symbiont of latrunculid sponges.bioRxiv : the preprint server for biology · 2024Article
- Article
- Dominant bacterial taxa drive microbiome differences of juvenile Pacific oysters of the same age and variable sizes.Frontiers in microbiomes · 2023Article
- Diversity of an uncommon elastic hypersaline microbial mat along a small-scale transect.PeerJ · 2022Article
- Spirochetes isolated from arthropods constitute a novel genus Entomospira genus novum within the order Spirochaetales.Scientific reports · 2020Article
- Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification ofFrontiers in microbiology · 2020Article
- Keeping it in the family: Coevolution of latrunculid sponges and their dominant bacterial symbionts.MicrobiologyOpen · 2017Article
- Characterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum.The ISME journal · 2016Article
- Abyssisolibacter fermentans gen. nov. sp. nov., isolated from deep sub-seafloor sediment.Journal of microbiology (Seoul, Korea) · 2016Article
- Planctomycetes do possess a peptidoglycan cell wall.Nature communications · 2015Article
- High-quality draft genome sequence of Gracilimonas tropica CL-CB462(T) (DSM 19535(T)), isolated from a Synechococcus culture.Standards in genomic sciences · 2015Article
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9 authors at 3 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
During a study of the anaerobic microbial community of a lithifying hypersaline microbial mat of Lake 21 on the Kiritimati atoll (Kiribati Republic, Central Pacific) strain L21-RPul-D2(T) was isolated. The closest phylogenetic neighbor was Spirochaeta africana Z-7692(T) that shared a 16S rRNA gene sequence identity value of 90% with the novel strain and thus was only distantly related. A comprehensive polyphasic study including determination of the complete genome sequence was initiated to characterize the novel isolate. Cells of strain L21-RPul-D2(T) had a size of 0.2 - 0.25 × 8-9 μm, were helical, motile, stained Gram-negative and produced an orange carotenoid-like pigment. Optimal conditions for growth were 35°C, a salinity of 50 g/l NaCl and a pH around 7.0. Preferred substrates for growth were carbohydrates and a few carboxylic acids. The novel strain had an obligate fermentative metabolism and produced ethanol, acetate, lactate, hydrogen and carbon dioxide during growth on glucose. Strain L21-RPul-D2(T) was aerotolerant, but oxygen did not stimulate growth. Major cellular fatty acids were C14:0, iso-C15:0, C16:0 and C18:0. The major polar lipids were an unidentified aminolipid, phosphatidylglycerol, an unidentified phospholipid and two unidentified glycolipids. Whole-cell hydrolysates contained L-ornithine as diagnostic diamino acid of the cell wall peptidoglycan. The complete genome sequence was determined and annotated. The genome comprised one circular chromosome with a size of 3.78 Mbp that contained 3450 protein-coding genes and 50 RNA genes, including 2 operons of ribosomal RNA genes. The DNA G + C content was determined from the genome sequence as 51.9 mol%. There were no predicted genes encoding cytochromes or enzymes responsible for the biosynthesis of respiratory lipoquinones. Based on significant differences to the uncultured type species of the genus Spirochaeta, S. plicatilis, as well as to any other phylogenetically related cultured species it is suggested to place strain L21-RPul-D2(T) (=DSM 27196(T) = JCM 18663(T)) in a novel species and genus, for which the name Salinispira pacifica gen. nov., sp. nov. is proposed.
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