ArticleNucleic acids research2015
A design principle for a single-stranded RNA genome that replicates with less double-strand formation.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Comparative insights into single stranded RNAs interaction with methylene blue and new methylene blue: a photophysical, thermodynamic and modeling study.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2026Article
- Screening metatranscriptomes for ultrastable RNA secondary structures reveals hidden bacteriophages and novel capsid nanomaterials.bioRxiv : the preprint server for biology · 2026Article
- Transforming RNA-Based Gene Therapy with Innovative Nanocarriers for siRNA and miRNA Delivery.Current gene therapy · 2026Review
- Mycoviruses: Antagonistic Potential, Fungal Pathogenesis, and Their Interaction withMicroorganisms · 2023Review
- Experimental evidence for the correlation between RNA structural fluctuations and the frequency of beneficial mutations.RNA (New York, N.Y.) · 2022Article
- In vitro characterisation of the MS2 RNA polymerase complex reveals host factors that modulate emesviral replicase activity.Communications biology · 2022Article
- Physicochemical Foundations of Life that Direct Evolution: Chance and Natural Selection are not Evolutionary Driving Forces.Life (Basel, Switzerland) · 2020Article
- Structural transition of replicable RNAs during in vitro evolution with Qβ replicase.RNA (New York, N.Y.) · 2020Article
- Viruses Infecting the Plant Pathogenic FungusViruses · 2019Review
- Cell-free expression of RNA encoded genes using MS2 replicase.Nucleic acids research · 2019Article
- Combinatorial selection for replicable RNA by Qβ replicase while maintaining encoded gene function.PloS one · 2017Article
- Constructive Approaches for Understanding the Origin of Self-Replication and Evolution.Life (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-stranded RNA (ssRNA) is the simplest form of genetic molecule and constitutes the genome in some viruses and presumably in primitive life-forms. However, an innate and unsolved problem regarding the ssRNA genome is formation of inactive double-stranded RNA (dsRNA) during replication. Here, we addressed this problem by focusing on the secondary structure. We systematically designed RNAs with various structures and observed dsRNA formation during replication using an RNA replicase (Qβ replicase). From the results, we extracted a simple rule regarding ssRNA genome replication with less dsRNA formation (less GC number in loops) and then designed an artificial RNA that encodes a domain of the β-galactosidase gene based on this rule. We also obtained evidence that this rule governs the natural genomes of all bacterial and most fungal viruses presently known. This study revealed one of the structural design principles of an ssRNA genome that replicates continuously with less dsRNA formation.
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