ArticleAnnals of biomedical engineering2016
Dielectrophoresis-Mediated Electrodeformation as a Means of Determining Individual Platelet Stiffness.
Article in Annals of biomedical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Differential regulation of GUV mechanics via actin network architectures.Biophysical journal · 2023Article
- Shear-Mediated Platelet Activation is Accompanied by Unique Alterations in Platelet Release of Lipids.Cellular and molecular bioengineering · 2021Article
- In Vitro Measurements of Shear-Mediated Platelet Adhesion Kinematics as Analyzed through Machine Learning.Annals of biomedical engineering · 2021Article
- Shear-mediated platelet activation in the free flow II: Evolving mechanobiological mechanisms reveal an identifiable signature of activation and a bi-directional platelet dyscrasia with thrombotic and bleeding features.Journal of biomechanics · 2021Review
- A predictive multiscale model for simulating flow-induced platelet activation: Correlating in silico results with in vitro results.Journal of biomechanics · 2021Article
- Electrophoretic transport and dynamic deformation of bio-vesicles.Electrophoresis · 2019Article
- An anti-clogging method for improving the performance and lifespan of blood plasma separation devices in real-time and continuous microfluidic systems.Scientific reports · 2018Article
- Routine clinical anti-platelet agents have limited efficacy in modulating hypershear-mediated platelet activation associated with mechanical circulatory support.Thrombosis research · 2018Article
- Erythrocyte Membrane Failure by Electromechanical Stress.Applied sciences (Basel, Switzerland) · 2018Article
- Characterization of biomechanical properties of cells through dielectrophoresis-based cell stretching and actin cytoskeleton modeling.Biomedical engineering online · 2017Article
- A multiscale biomechanical model of platelets: Correlating with in-vitro results.Journal of biomechanics · 2017Article
- Shear-mediated platelet activation in the free flow: Perspectives on the emerging spectrum of cell mechanobiological mechanisms mediating cardiovascular implant thrombosis.Journal of biomechanics · 2017Article
- Flow-Induced Damage to Blood Cells in Aortic Valve Stenosis.Annals of biomedical engineering · 2016Article
- Lagrangian methods for blood damage estimation in cardiovascular devices--How numerical implementation affects the results.Expert review of medical devices · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Platelets, essential for hemostasis, are easily activated via biochemical and mechanical stimuli. Cell stiffness is a vital parameter modulating the mechano-transduction of exogenous mechanical stimuli. While methods exist to measure cell stiffness, no ready method exists for measuring platelet stiffness that is both minimally-contacting, imparting minimal exogenous force and non-activating. We developed a minimal-contact methodology capable of trapping and measuring the stiffness of individual platelets utilizing dielectrophoresis (DEP)-mediated electrodeformation. Parametric studies demonstrate a non-uniform electric field in the MHz frequency range (0.2-20 MHz) is required for generating effective DEP forces on platelets, suspended in isotonic buffer with conductivity ~100-200 μS/cm. A nano-Newton DEP force (0.125-4.5 nN) was demonstrated to be essential for platelet electrodeformation, which could be generated with an electric field with strength of 1.5-9 V/μm. Young's moduli of platelets were calculated using a Maxwell stress tensor model and stress-deformation relationship. Platelet stiffness was determined to be in the range of 3.5 ± 1.4 and 8.5 ± 1.5 kPa for resting and 0.4% paraformaldehyde-treated cells, respectively. The developed methodology fills a gap in approaches of measuring individual platelet stiffness, free of inadvertent platelet activation, which will facilitate further studies of mechanisms involved in mechanically-mediated platelet activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.