Evidence map›Paper›PMID 26202303›Full record

ArticleIn vitro cellular & developmental biology. Animal2015

Isolation and purification of rabbit mesenchymal stem cells using an optimized protocol.

Chunbo Lin, Maorong Shen, Weiping Chen, Xiaofeng Li, Daoming Luo, Jinhong Cai, Yuan Yang

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Chunbo LinGuangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
Maorong ShenGuangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
Weiping ChenGuangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China.
Xiaofeng LiGuangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
Daoming LuoGuangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
Jinhong CaiGuangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China.
Yuan YangGuangxi Orthopedics Traumatology Hospital, Nanning, Guangxi Zhuang Autonomous Region, China. yangy062@sina.com.
Guangxi Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stem cells were first isolated and grown in vitro by Friedenstein over 40 yr ago; however, their isolation remains challenging as they lack unique markers for identification and are present in very small quantities in mesenchymal tissues and bone marrow. Using whole marrow samples, common methods for mesenchymal stem cell isolation are the adhesion method and density gradient fractionation. The whole marrow sample adhesion method still results in the nonspecific isolation of mononuclear cells, and activation and/or potential loss of target cells. Density gradient fractionation methods are complicated, and may result in contamination with toxic substances that affect cell viability. In the present study, we developed an optimized protocol for the isolation and purification of mesenchymal stem cells based on the principles of hypotonic lysis and natural sedimentation.

Indexed as

AdipocytesAdipogenesisAnimalsBone MarrowBone Marrow CellsCell Culture TechniquesCell DifferentiationCell ProliferationCell SeparationCentrifugationCulture MediaMaleMesenchymal Stem CellsOsteocytesOsteogenesisRabbitsCulture MediaHypotonic lysisIsolationMesenchymal stem cellsNatural sedimentationPurification

Identifiers

PMID26202303
OpenAlexW1435532891

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.