Evidence map›Paper›PMID 26202258›Full record

ArticleJournal of molecular neuroscience : MN2015

PACAP Modulates Expression of Hypoxia-Inducible Factors in Streptozotocin-Induced Diabetic Rat Retina.

Agata Grazia D'Amico, Grazia Maugeri, Rita Reitano, Claudio Bucolo, Salvatore Saccone, Filippo Drago, Velia D'Agata

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In one paragraph

Article in Journal of molecular neuroscience : MN, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.

  1. Matrix metalloproteinase 14 modulates diabetes and Alzheimer's disease cross-talk: a meta-analysis.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2018
    Pooled it
  2. Review
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  4. Article
  5. Review
  6. Article
  7. Protective Effects of PACAP in a Rat Model of Diabetic Neuropathy.International journal of molecular sciences · 2021
    Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Review
  17. Neuroprotective Peptides in Retinal Disease.Journal of clinical medicine · 2019
    Review
  18. Review
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  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Agata Grazia D'AmicoSection of Human Anatomy and Histology, University of Catania, Catania, Italy.
Grazia MaugeriSection of Human Anatomy and Histology, University of Catania, Catania, Italy.
Rita ReitanoSection of Human Anatomy and Histology, University of Catania, Catania, Italy.
Claudio BucoloSection of Pharmacology Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
Salvatore SacconeSection of Animal Biology, Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy.
Filippo DragoSection of Pharmacology Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
Velia D'AgataSection of Human Anatomy and Histology, University of Catania, Catania, Italy. vdagata@unict.it.
University of Catania · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Retinal hypoxia has been related to the pathogenesis of diabetic retinopathy. This event is mediated by the hypoxia-inducible factors (HIFs), including HIF-1α, HIF-2α, and HIF-3α. Previously, we have demonstrated the protective role of pituitary adenylate cyclase-activating peptide (PACAP) in the early phase of diabetic retinopathy. In the present work, we investigated whether PACAP effect in hyperglycemic retina is mediated through modulation of HIFs' expression. Diabetes was induced with a single injection of streptozotocin (STZ) in rats. After 1 week, a group of diabetic animals was treated with a single intravitreal injection of 100 μM PACAP or saline solution. Then, changes in HIFs' expression levels were evaluated in the retina after 3 weeks of hyperglycemia. The expression of HIF-1α and HIF-2α was significantly (p < 0.001 vs control) increased in diabetic rats as compared to controls. Instead, their expression levels were significantly (p < 0.001 vs STZ) decreased after PACAP intraocular administration, as detected by Western blot analysis. Conversely, the expression of HIF-3α was significantly (p < 0.001 vs control) downregulated in retinas of STZ-injected rats and significantly (p < 0.001 vs control) increased after PACAP treatment. These data were supported by the immunohistochemical analysis. HIFs were localized either in inner and outer retinal layers. Diabetes interferes with their distribution, which is changed following intravitreal injection of PACAP. The present results suggest that the protective effect of the peptide in diabetic retina might be also mediated through modulation of HIFs' expression.

Indexed as

AnimalsBasic Helix-Loop-Helix ProteinsDiabetic RetinopathyEndothelial PAS Domain-Containing Protein 1Hypoxia-Inducible Factor 1Intravitreal InjectionsMalePituitary Adenylate Cyclase-Activating PolypeptideRatsRats, Sprague-DawleyRetinaBasic Helix-Loop-Helix ProteinsEndothelial PAS Domain-Containing Protein 1Hypoxia-Inducible Factor 1Pituitary Adenylate Cyclase-Activating PolypeptideDiabetic retinopathyHypoxia-inducible factorsPACAPSTZ-injected rats

Identifiers

PMID26202258
OpenAlexW956681885

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.