ArticleJournal of clinical microbiology2015
Characterization of Hepatitis C Virus Recombination in Cameroon by Use of Nonspecific Next-Generation Sequencing.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Mixed Infections Unravel Novel HCV Inter-Genotypic Recombinant Forms within the Conserved IRES Region.Viruses · 2024Article
- DAA Treatment Failure in a HIV/HBV/HCV Co-Infected Patient Carrying a Chimeric HCV Genotype 4/1b.International journal of environmental research and public health · 2022Article
- Global Patterns of Recombination across Human Viruses.Molecular biology and evolution · 2021Article
- Validation of Variant Assembly Using HAPHPIPE with Next-Generation Sequence Data from Viruses.Viruses · 2020Article
- Hepatitis C virus genotyping based on Core and NS5B regions in Cameroonian patients.Virology journal · 2019Article
- Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.PLoS neglected tropical diseases · 2019Article
- Highly Diverse Hepatitis C Strains Detected in Sub-Saharan Africa Have Unknown Susceptibility to Direct-Acting Antiviral Treatments.Hepatology (Baltimore, Md.) · 2019Article
- Hepatitis C virus surveillance and identification of human pegivirus 2 in a large Cameroonian cohort.Journal of viral hepatitis · 2019Article
- Genetic characterization of norovirus GII.4 variants circulating in Canada using a metagenomic technique.BMC infectious diseases · 2018Article
- Barley RNA viromes in six different geographical regions in Korea.Scientific reports · 2018Article
- Superinfection and cure of infected cells as mechanisms for hepatitis C virus adaptation and persistence.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Low prevalence of HCV infection with predominance of genotype 4 among HIV patients living in Libreville, Gabon.PloS one · 2018Article
- Epidemiology of viral hepatitis in the Republic of Congo: review.BMC research notes · 2017Review
- Characterization of the Genomic Diversity of Norovirus in Linked Patients Using a Metagenomic Deep Sequencing Approach.Frontiers in microbiology · 2017Article
- Genetic diversity of hepatitis C virus in Ethiopia.PloS one · 2017Article
- Hepatitis C in Cameroon: What is the progress from 2001 to 2016?Journal of translational internal medicine · 2016Article
- ve-SEQ: Robust, unbiased enrichment for streamlined detection and whole-genome sequencing of HCV and other highly diverse pathogens.F1000Research · 2015Article
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Authors and funding
11 authors.
Funding
Abstract
The importance of recombination in the evolution and genetic diversity of the hepatitis C virus (HCV) is currently uncertain. Only a small number of intergenotypic recombinants have been identified so far, and each has core and envelope genes classified as belonging to genotype 2. Here, we investigated two putative genotype 4/1 recombinants from southern Cameroon using a number of approaches, including standard Sanger sequencing, genotype-specific PCR amplification, and non-HCV-specific Illumina RNA sequencing (RNA-seq). Recombination between genotypes 1 and 4 was confirmed in both samples, and the parental lineages of each recombinant belong to HCV subtypes that are cocirculating at a high prevalence in Cameroon. Using the RNA-seq approach, we obtained a complete genome for one sample, which contained a recombination breakpoint at the E2/P7 gene junction. We developed and applied a new method, called Deep SimPlot, which can be used to visualize and identify viral recombination directly from the short sequence reads created by next-generation sequencing in conjunction with a consensus sequence.
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