ArticleScientific reports2015
Compaction of rolling circle amplification products increases signal integrity and signal-to-noise ratio.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
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- Tethered Catalytic Hairpin Assembly with Plasmon-Enhanced Fluorescence Readout for Single Molecule Detection.Small methods · 2025Article
- Digital assay for rapid electronic quantification of clinical pathogens using DNA nanoballs.Science advances · 2023Article
- Simultaneous detection of miRNA and mRNA at the single-cell level in plant tissues.Plant biotechnology journal · 2023Article
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- Rolling Circle Amplification in Integrated Microsystems: An Uncut Gem toward Massively Multiplexed Pathogen Diagnostics and Genotyping.Accounts of chemical research · 2021Review
- In situ genome sequencing resolves DNA sequence and structure in intact biological samples.Science (New York, N.Y.) · 2021Article
- Mitochondrial dysfunction in sepsis is associated with diminished intramitochondrial TFAM despite its increased cellular expression.Scientific reports · 2020Observational
- A multiplex platform for digital measurement of circular DNA reaction products.Nucleic acids research · 2020Article
- Integration of microbead DNA handling with optomagnetic detection in rolling circle amplification assays.Mikrochimica acta · 2019Article
- HiPLA: High-throughput imaging proximity ligation assay.Methods (San Diego, Calif.) · 2019Article
- Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes.Scientific reports · 2018Article
- Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy.Nature communications · 2017Article
- Let There Be Light!Proteomes · 2016Review
- Intronless WNT10B-short variant underlies new recurrent allele-specific rearrangement in acute myeloid leukaemia.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rolling circle amplification (RCA) for generation of distinct fluorescent signals in situ relies upon the self-collapsing properties of single-stranded DNA in commonly used RCA-based methods. By introducing a cross-hybridizing DNA oligonucleotide during rolling circle amplification, we demonstrate that the fluorophore-labeled RCA products (RCPs) become smaller. The reduced size of RCPs increases the local concentration of fluorophores and as a result, the signal intensity increases together with the signal-to-noise ratio. Furthermore, we have found that RCPs sometimes tend to disintegrate and may be recorded as several RCPs, a trait that is prevented with our cross-hybridizing DNA oligonucleotide. These effects generated by compaction of RCPs improve accuracy of visual as well as automated in situ analysis for RCA based methods, such as proximity ligation assays (PLA) and padlock probes.
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