Evidence map›Paper›PMID 26200868›Full record

ArticleBiophysical journal2015

DMSO induces dehydration near lipid membrane surfaces.

Chi-Yuan Cheng, Jinsuk Song, Jolien Pas, Lenny H H Meijer, Songi Han

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 108 citations in OpenAlex.

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  13. Central Carbon Metabolism inJournal of fungi (Basel, Switzerland) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Chi-Yuan ChengDepartment of Chemistry and Biochemistry, University of California, Santa Barbara, Santa Barbara, California.
Jinsuk SongDepartment of Chemistry and Biochemistry, University of California, Santa Barbara, Santa Barbara, California.
Jolien PasDepartment of Biomedical Engineering, Laboratory of Chemical Biology and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands.
Lenny H H MeijerDepartment of Biomedical Engineering, Laboratory of Chemical Biology and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands.
Songi HanDepartment of Chemistry and Biochemistry, University of California, Santa Barbara, Santa Barbara, California. Electronic address: songi@chem.ucsb.edu.
University of California, Santa Barbara · USEindhoven University of Technology · NL

Funding

Probing early protein aggregation mechanisms and their relationship to disease efDP2OD008702 · OD · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI HAN, SONGI · 2011 to 2011
$2.3M
NIH HHS DP2 OD008702
6 · The paper itself

Abstract

Dimethyl sulfoxide (DMSO) has been broadly used in biology as a cosolvent, a cryoprotectant, and an enhancer of membrane permeability, leading to the general assumption that DMSO-induced structural changes in cell membranes and their hydration water play important functional roles. Although the effects of DMSO on the membrane structure and the headgroup dehydration have been extensively studied, the mechanism by which DMSO invokes its effect on lipid membranes and the direct role of water in this process are unresolved. By directly probing the translational water diffusivity near unconfined lipid vesicle surfaces, the lipid headgroup mobility, and the repeat distances in multilamellar vesicles, we found that DMSO exclusively weakens the surface water network near the lipid membrane at a bulk DMSO mole fraction (XDMSO) of <0.1, regardless of the lipid composition and the lipid phase. Specifically, DMSO was found to effectively destabilize the hydration water structure at the lipid membrane surface at XDMSO <0.1, lower the energetic barrier to dehydrate this surface water, whose displacement otherwise requires a higher activation energy, consequently yielding compressed interbilayer distances in multilamellar vesicles at equilibrium with unaltered bilayer thicknesses. At XDMSO >0.1, DMSO enters the lipid interface and restricts the lipid headgroup motion. We postulate that DMSO acts as an efficient cryoprotectant even at low concentrations by exclusively disrupting the water network near the lipid membrane surface, weakening the cohesion between water and adhesion of water to the lipid headgroups, and so mitigating the stress induced by the volume change of water during freeze-thaw.

Indexed as

Membranes, Artificial1,2-DipalmitoylphosphatidylcholineDiffusionDimethyl SulfoxideFatty Acids, MonounsaturatedMagnetic Resonance SpectroscopyPhosphatidylcholinesPhosphatidylglycerolsQuaternary Ammonium CompoundsScattering, Small AngleWaterX-Ray Diffraction1,2-dioleoyloxy-3-(trimethylammonium)propane1,2-Dipalmitoylphosphatidylcholine1,2-dipalmitoylphosphatidylglycerol1,2-oleoylphosphatidylcholinecolfosceril palmitateDimethyl SulfoxideFatty Acids, MonounsaturatedMembranes, ArtificialPhosphatidylcholinesPhosphatidylglycerolsQuaternary Ammonium CompoundsWater

Identifiers

PMID26200868
PMCPMC4621616
OpenAlexW1834124027

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.