ArticleJournal of applied oral science : revista FOB
A new improved protocol for in vitro intratubular dentinal bacterial contamination for antimicrobial endodontic tests: standardization and validation by confocal laser scanning microscopy.
Article in Journal of applied oral science : revista FOB. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 65 citations in OpenAlex.
- Impact of EDTA Formulations and Activation Methods on Stem Cell Response, Antimicrobial Activity and Dentine Integrity: An In Vitro Study.Australian endodontic journal : the journal of the Australian Society of Endodontology Inc · 2026Article
- Chemical and antimicrobial profiling of glycolic and etidronic acid as endodontic irrigants.Odontology · 2026Article
- Physicochemical stability and bactericidal efficacy of a novel premixed triple antibiotic paste during storage: an in vitro study.BMC oral health · 2026Article
- Biological, antimicrobial, and apical tissue dissolution effects of ambroxol hydrochloride as an irrigant for regenerative endodontics: an in vitro study.BMC oral health · 2026Article
- Antimicrobial Effectiveness of High-Power Sonic and Ultrasonic Devices Combined with Stepwise Intraoperative or Final Activation of Sodium Hypochlorite.European endodontic journal · 2025Article
- Are ultrasonic tips associated with final irrigation protocols effective in removing biofilms in long oval canals and dentinal tubules?Clinical oral investigations · 2025Article
- In vitro disinfection, pH and solubility of bioceramic intracanal medication with calcium hydroxide.Brazilian oral research · 2025Article
- Bactericidal Effect of Triple Antibiotic Paste against Enterococcus faecalis in Dentinal Tubules: An Ex Vivo Study.European endodontic journal · 2024Article
- Sonic-assisted antibacterial photodynamic therapy: a strategy for enhancing lateral canal disinfection.BMC oral health · 2024Article
- Reducing Apical Bacterial Extrusion: The Impact of Reciproc File Size and Irrigation Technique.Iranian endodontic journal · 2024Article
- Antimicrobial efficacy of sodium hypochlorite and hyper-pure chlorine dioxide in the depth of dentin tubules in vitro.BMC oral health · 2023Article
- A new model ofHeliyon · 2023Article
- The Vehicles of Calcium Hydroxide Pastes Interfere with Antimicrobial Effect, Biofilm Polysaccharidic Matrix, and Pastes' Physicochemical Properties.Biomedicines · 2022Article
- Can the Concentration of Citric Acid Affect Its Cytotoxicity and Antimicrobial Activity?Dentistry journal · 2022Article
- Ultrasonic agitation reduces the time of calcium hydroxide antimicrobial effect and enhances its penetrability.Journal of materials science. Materials in medicine · 2021Article
- Antibacterial efficacy of copper-added chitosan nanoparticles: a confocal laser scanning microscopy analysis.Odontology · 2021Article
- Chemical and mechanical influence of root canal irrigation on biofilm removal from lateral morphological features of simulated root canals, dentine discs and dentinal tubules.International endodontic journal · 2021Article
- Antibacterial Effect of Photodynamic Therapy on Root Canal Disinfection Combined with Different Irrigation Protocols.Iranian endodontic journal · 2020Article
- Article
- Quantitative Assessment of Dentinal Tubule Disinfection in Absence of Biofilm on Root Canal Walls: AnIranian endodontic journal · 2020Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo compare three methods of intratubular contamination that simulate endodontic infections using confocal laser scanning microscopy (CLSM). MATERIAL AND
methodsTwo pre-existing models of dentinal contamination were used to induce intratubular infection (groups A and B). These methods were modified in an attempt to improve the model (group C). Among the modifications it may be included: specimen contamination for five days, ultrasonic bath with BHI broth after specimen sterilization, use of E. faecalisduring the exponential growth phase, greater concentration of inoculum, and two cycles of centrifugation on alternate days with changes of culture media. All specimens were longitudinally sectioned and stained with of LIVE/DEAD for 20 min. Specimens were assessed using CLSM, which provided images of the depth of viable bacterial proliferation inside the dentinal tubules. Additionally, three examiners used scores to classify the CLSM images according to the following parameters: homogeneity, density, and depth of the bacterial contamination inside the dentinal tubules. Kruskal-Wallis and Dunn's tests were used to evaluate the live and dead cells rates, and the scores obtained.
resultsThe contamination scores revealed higher contamination levels in group C when compared with groups A and B (p<0.05). No differences were observed between group A and B (p>0.05). The volume of live cells in group C was higher than in groups A and B (p<0.05).
conclusionThe new protocol for intratubular infection resulted in high and uniform patterns of bacterial contamination and higher cell viability in all specimens when compared with the current methods.
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