ArticlePloS one2015
Overproduction of a Model Sec- and Tat-Dependent Secretory Protein Elicits Different Cellular Responses in Streptomyces lividans.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- Review
- Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus.Microbial cell factories · 2019Article
- Functional identification of aOpen biology · 2019Article
- Dynamic metabolic modelling of overproduced protein secretion in Streptomyces lividans using adaptive DFBA.BMC microbiology · 2019Article
- Four thiol-oxidoreductases involved in the formation of disulphide bonds in the Streptomyces lividans TK21 secretory proteins.Microbial cell factories · 2019Article
- Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein.Microbial cell factories · 2018Article
- Modelling the metabolism of protein secretion through the Tat route in Streptomyces lividans.BMC microbiology · 2018Article
- The Cellular Mechanisms that Ensure an Efficient Secretion in Streptomyces.Antibiotics (Basel, Switzerland) · 2018Review
- Monitoring Protein Secretion inFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptomyces lividans is considered an efficient host for the secretory production of homologous and heterologous proteins. To identify possible bottlenecks in the protein production process, a comparative transcriptomic approach was adopted to study cellular responses during the overproduction of a Sec-dependent model protein (alpha-amylase) and a Tat-dependent model protein (agarase) in Streptomyces lividans. The overproduction of the model secretory proteins via the Sec or the Tat route in S. lividans does elicit a different major cell response in the bacterium. The stringent response is a bacterial response to nutrients' depletion, which naturally occurs at late times of the bacterial cell growth. While the induction of the stringent response at the exponential phase of growth may limit overall productivity in the case of the Tat route, the induction of that response does not take place in the case of the Sec route, which comparatively is an advantage in secretory protein production processes. Hence, this study identifies a potential major drawback in the secretory protein production process depending on the secretory route, and provides clues to improving S. lividans as a protein production host.
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