ArticleLab animal2015
Effects of different blood collection methods on indicators of welfare in mice.
Article in Lab animal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Comparable immunogenicity from murine blood collection methods in intranasal gonococcal vaccination with ACP and MtrE supports refinement of preclinical vaccine studies.Scientific reports · 2026Article
- Exploring rodent prosociality: A conceptual framework.Translational neuroscience · 2025Review
- A new external jugular venipuncture technique for efficient vascular access that exploits a murine anatomical variation.PloS one · 2025Article
- Article
- Glucose-independent insulin and glucagon secreted from ventral pancreas sharing a similar pattern in healthy adult rat.Veterinary research forum : an international quarterly journal · 2022Article
- A Comparison of Blood Collection Techniques in Mice and their Effects on Welfare.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Article
- Improving the Quality of EDTA-treated Blood Specimens from Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Article
- Research-Relevant Conditions and Pathology of Laboratory Mice, Rats, Gerbils, Guinea Pigs, Hamsters, Naked Mole Rats, and Rabbits.ILAR journal · 2021Review
- Comparison of murine retroorbital plexus and facial vein blood collection to mitigate animal ethics issues.Laboratory animal research · 2021Article
- Recent Advances in Experimental Models of Breast Cancer Exosome Secretion, Characterization and Function.Journal of mammary gland biology and neoplasia · 2020Review
- Stress and behavioral correlates in the head-fixed method: stress measurements, habituation dynamics, locomotion, and motor-skill learning in mice.Scientific reports · 2020Article
- Behavioral Methods for Severity Assessment.Animals : an open access journal from MDPI · 2020Review
- The Impact of Common Recovery Blood Sampling Methods, in Mice (Mus Musculus), on Well-Being and Sample Quality: A Systematic Review.Animals : an open access journal from MDPI · 2020Review
- Impact of three commonly used blood sampling techniques on the welfare of laboratory mice: Taking the animal's perspective.PloS one · 2020Article
- Time-dependent Pathologic and Inflammatory Consequences of Various Blood Sampling Techniques in Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- A minimally invasive, low-stress method for serial blood collection in aging mice.Pathobiology of aging & age related diseases · 2019Article
- Comparison of Serial Blood Collection by Facial Vein and Retrobulbar Methods in C57BL/6 Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2018Article
- Comparing Phlebotomy by Tail Tip Amputation, Facial Vein Puncture, and Tail Vein Incision in C57BL/6 Mice by Using Physiologic and Behavioral Metrics of Pain and Distress.Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Sex differences in variability across timescales in BALB/c mice.Biology of sex differences · 2017Article
- Comparison of Submental Blood Collection with the Retroorbital and Submandibular Methods in Mice (Mus musculus).Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blood collection is a common experimental procedure for which there are many different methods, each with its own advantages and disadvantages. Researchers should use methods that minimize pain, suffering, distress and lasting harm to animals while meeting study requirements. The authors evaluated stress, activity and tissue damage in BALB/cO1aHsd mice after collecting blood using one of six methods: retrobulbar bleeding with thin or thick capillaries, tail vein bleeding, saphenous vein bleeding, facial vein bleeding or jugular vein bleeding. The authors compared in-cage activity, corticosterone concentration and performance in open-field tests between treatment groups and collected histologic samples at 1 h, 3 d and 14 d after bleeding. Mice that underwent retrobulbar bleeding with a thick capillary had a smaller change in corticosterone concentration and higher in-cage activity immediately after blood collection, whereas mice that underwent jugular vein bleeding had a greater change in corticosterone concentration and lower in-cage activity and open-field activity. Mice that underwent saphenous vein bleeding had a high incidence of histological change at 1 h, 3 d and 14 d after blood collection, but few indicators of histological change were present in other groups at 14 d after blood collection. These results suggest that, when collecting a small volume of blood, retrobulbar bleeding with a thick capillary and without anesthesia causes the least stress in mice, whereas jugular vein bleeding and facial vein bleeding cause the most stress and saphenous vein bleeding causes the most lasting damage in mice.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.