ArticleProceedings of the National Academy of Sciences of the United States of America2015
Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
17 citing papers in PubMed.
- ProteinMPNN Recovers Complex Sequence Properties of Transmembrane β-barrels.bioRxiv : the preprint server for biology · 2024Article
- The Name Is Barrel, β-Barrel.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Evolutionary Engineering a Larger Porin Using a Loop-to-Hairpin Mechanism.Journal of molecular biology · 2023Article
- Evolutionary engineering a larger porin using a loop-to-hairpin mechanism.bioRxiv : the preprint server for biology · 2023Article
- Protein Design: From the Aspect of Water Solubility and Stability.Chemical reviews · 2022Review
- Dawn of a New Era for Membrane Protein Design.Biodesign research · 2022Review
- Outer membrane protein evolution.Current opinion in structural biology · 2021Review
- De novo design of transmembrane β barrels.Science (New York, N.Y.) · 2021Article
- Water in Nanopores and Biological Channels: A Molecular Simulation Perspective.Chemical reviews · 2020Review
- Designing minimalist membrane proteins.Biochemical Society transactions · 2019Review
- Building bigger beta-barrels.eLife · 2019Article
- Article
- Th2-biased immune response and agglutinating antibodies generation by a chimeric protein comprising OmpC epitope (323-336) of Aeromonas hydrophila and LTB.Immunologic research · 2018Article
- Outer membrane protein folding from an energy landscape perspective.BMC biology · 2017Review
- Outer membrane protein design.Current opinion in structural biology · 2017Review
- Searching for the Pareto frontier in multi-objective protein design.Biophysical reviews · 2017Review
- Engineering a Novel Porin OmpGF Via Strand Replacement from Computational Analysis of Sequence Motif.Biochimica et biophysica acta. Biomembranes · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Advances in computational design methods have made possible extensive engineering of soluble proteins, but designed β-barrel membrane proteins await improvements in our understanding of the sequence determinants of folding and stability. A subset of the amino acid residues of membrane proteins interact with the cell membrane, and the design rules that govern this lipid-facing surface are poorly understood. We applied a residue-level depth potential for β-barrel membrane proteins to the complete redesign of the lipid-facing surface of Escherichia coli OmpA. Initial designs failed to fold correctly, but reversion of a small number of mutations indicated by backcross experiments yielded designs with substitutions to up to 60% of the surface that did support folding and membrane insertion.
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Registered trials
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