Evidence map›Paper›PMID 26198909›Full record

ArticleInternational endodontic journal2016

The dental pulp stem cell niche based on aldehyde dehydrogenase 1 expression.

C V Machado, S T Passos, T M C Campos, L Bernardi, D S Vilas-Bôas, J E Nör, P D S Telles, I L Nascimento

Abstract read
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In one paragraph

Article in International endodontic journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

C V MachadoDepartment of Bio-Interaction, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
S T PassosDepartment of Bio-Interaction, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
T M C CamposDepartment of Bio-Interaction, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
L BernardiDepartment of Bio-Interaction, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
D S Vilas-BôasDepartment of Bio-Interaction, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
J E NörDepartment of Bio-Interaction, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
P D S TellesDepartment of Bio-Interaction, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
I L NascimentoDepartment of Bio-Interaction, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
Universidade Federal da Bahia · BR

Funding

Mechanisms of dental pulp stem cell differentiation into functional endotheliumR01DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NOR, JACQUES EDUARDO · 2011 to 2021
$3.2M
Regulation of dental pulp stem cell fateR37DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Jacques Eduardo Nor · 2024 to 2026
$1.2M
NIDCR NIH HHS R01 DE021410NIDCR NIH HHS R37 DE021410
6 · The paper itself

Abstract

aimTo detect cells expressing the stem cell marker ALDH1 (aldehyde dehydrogenase1) in the pulp of human permanent teeth and to investigate the expression of ALDH1 in isolated dental pulp cells. METHODOLOGY: Pulp tissue was collected and processed for immunohistochemistry to detect ALDH1-, STRO-1- and CD90-positive cells. In addition, cells were isolated and analysed by flow cytometry for ALDH1 activity and for the cell surface markers CD44, CD73, CD90, STRO-1 and CD45. Cells were also examined for multidifferentiation capacity. Within these cells, an ALDH1(+) cell subpopulation was selected and evaluated for multidifferentiation capacity.

resultsThe immunohistochemistry analyses showed that ALDH1-, CD90- and STRO-1-positive cells were located mainly in the perivascular areas and nerve fibres of dental pulps. Cells on the fifth passage had high expression for CD44, CD73 and CD90, whereas moderate labelling was observed for STRO-1 and ALDH1 in flow cytometry analysis. On the same passages, cells were able to differentiate into osteogenic, adipogenic and chondrogenic lineages. The ALDH1(+) cell subpopulation also demonstrated multilineage differentiation ability.

conclusionsDental pulp stem cells reside in the vicinity of blood vessels and nerve fibres, indicating the possible existence of more than one stem cell niche in dental pulps. Furthermore, ALDH1 was expressed by isolated dental pulp cells, which had mesenchymal stem cell characteristics. Thus, it can be suggested that ALDH1 may be used as a DPSC marker.

Indexed as

Stem CellsAdolescentAdultAldehyde Dehydrogenase 1 FamilyAntigens, SurfaceBiomarkersBlood VesselsDental PulpHumansIsoenzymesMolar, ThirdRetinal DehydrogenaseThy-1 AntigensYoung AdultAldehyde Dehydrogenase 1 FamilyALDH1A1 protein, humanAntigens, SurfaceBiomarkersIsoenzymesRetinal DehydrogenaseSTRO-1 antigen, humanThy-1 Antigensaldehyde dehydrogenase 1dental pulpstem cell nichestem cells

Identifiers

PMID26198909
PMCPMC4723291
OpenAlexW2117521358

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.