ArticleBMC biotechnology2015
Expression of dsRNA in recombinant Isaria fumosorosea strain targets the TLR7 gene in Bemisia tabaci.
Article in BMC biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 87 citations in OpenAlex.
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- Enhancing the Virulence of a Fungal Entomopathogen Against the Brown Planthopper by Expressing dsRNA to Suppress Host Immune Defenses.Microorganisms · 2025Article
- Targeting vacuolar ATPase in tobacco whitefly,Journal of biosciences · 2025Review
- Spray-induced gene silencing for crop protection: recent advances and emerging trends.Frontiers in plant science · 2025Review
- Exogenous Application of dsRNA in Plant Protection: Efficiency, Safety Concerns and Risk Assessment.International journal of molecular sciences · 2024Review
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- Cysteine facilitates the lignocellulolytic response of Trichoderma guizhouense NJAU4742 by indirectly up-regulating membrane sugar transporters.Biotechnology for biofuels and bioproducts · 2023Article
- A life-and-death struggle: interaction of insects with entomopathogenic fungi across various infection stages.Frontiers in immunology · 2023Review
- Characterization and transcriptomic analysis of a native fungal pathogen against the rice pestFrontiers in microbiology · 2023Article
- Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application.International journal of molecular sciences · 2022Review
- RNA meets toxicology: efficacy indicators from the experimental design of RNAi studies for insect pest management.Pest management science · 2022Review
- Current developments in the resistance, quality, and production of entomopathogenic fungi.World journal of microbiology & biotechnology · 2022Review
- Combatting insects mediated biotic stress through plant associated endophytic entomopathogenic fungi in horticultural crops.Frontiers in plant science · 2022Review
- General Limitations to Endophytic Entomopathogenic Fungi Use as Plant Growth Promoters, Pests and Pathogens Biocontrol Agents.Plants (Basel, Switzerland) · 2021Review
- Review
- Phototoxicity of Ultraviolet-A against the WhiteflyOxidative medicine and cellular longevity · 2021Article
- Advances in the Development of Microbial Double-Stranded RNA Production Systems for Application of RNA Interference in Agricultural Pest Control.Frontiers in bioengineering and biotechnology · 2021Review
- Model Application of Entomopathogenic Fungi as Alternatives to Chemical Pesticides: Prospects, Challenges, and Insights for Next-Generation Sustainable Agriculture.Frontiers in plant science · 2021Review
- RNA Interference-Based Forest Protection Products (FPPs) Against Wood-Boring Coleopterans: Hope or Hype?Frontiers in plant science · 2021Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA interference (RNAi) technology shows a great potential in controlling agricultural pests, despite the difficulty of introducing exogenous dsRNA/siRNA into target pests. Isaria fumosorosea is a common fungal pathogen of the B-biotype Bemisia tabaci (whitefly), which is a widespread pest. Entomopathogenic fungi directly penetrate the cuticle and invade insect hemocoel. Application of I. fumosorosea expressing dsRNA of whitefly immunity-related gene may aid in developing RNAi technology to effectively control whiteflies.
methodsA dsRNA expression plasmid, psTLR7, was constructed by introducing the Toll-like receptor 7 (TLR7) gene of B-biotype whitefly to the silent vector, pSilent-1. The plasmid psTLR7 was transferred into the protoplast of the I. fumosorosea strain IfB01. Then, the recombinant strain was screened out based on the biological stability and bioactivity against whitefly.
resultsA genetically stable recombinant strain IfB01-TRL7 was screened out. The impact of IfB01-TRL7 against whitefly TRL7 gene was validated by qPCR. Lower expression levels of the TLR7 gene was observed in the whiteflies infected by the recombinant strain. The bioassay results indicated that compared to IfB01 strain, IfB01-TRL7 increased the mortality of whitefly nymphs, and decreased and shortened the values of LC50 and LT50, thus indicating higher virulence of IfB01-TRL7.
conclusionThe expression of the dsRNA of whitefly TLR7 gene in recombinant I. fumosorosea strain successfully knocked down the host target gene by infecting the nymphs and enhanced the whiteflies mortality. The present study will give insight to new application of RNAi technology for more effective biocontrol of this pests.
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