ArticleExtremophiles : life under extreme conditions2015
Medium-based optimization of an organic solvent-tolerant extracellular lipase from the isolated halophilic Alkalibacillus salilacus.
Article in Extremophiles : life under extreme conditions, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- Production of alkaline lipase by Aspergillus terreus AUMC 15762 for laundry application.AMB Express · 2025Article
- Expression of microbial lipase in filamentous fungus3 Biotech · 2024Review
- A thermostable organic solvent-tolerant lipase fromFrontiers in microbiology · 2023Article
- Review
- Elimination and detoxification of phenanthrene assisted by a laccase from halophileJournal of environmental health science & engineering · 2022Article
- Bioremoval and Detoxification of Anthracene by a Halophilic Laccase fromIranian journal of biotechnology · 2022Article
- High Salinity Inhibits Soil Bacterial Community Mediating Nitrogen Cycling.Applied and environmental microbiology · 2021Article
- Production strategies and biotechnological relevance of microbial lipases: a review.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Exoenzyme-producing halophilic bacteria from the former Lake Texcoco: identification and production of n-butyl oleate and bioactive peptides.Folia microbiologica · 2020Article
- Enhancement of Aeribacillus pallidus strain VP3 lipase catalytic activity through optimization of medium composition using Box-Behnken design and its application in detergent formulations.Environmental science and pollution research international · 2020Article
- Biosynthesis of butyl esters from crude oil of palm fruit and kernel using halophilic lipase secretion by3 Biotech · 2019Article
- Statistical optimization of cultural medium composition of thermoalkalophilic lipase produced by a chemically induced mutant strain of3 Biotech · 2019Article
- Efficient Keratinolysis of Poultry Feather Waste by the Halotolerant Keratinase from Salicola Marasensis.Iranian journal of pharmaceutical research : IJPR · 2019Article
- The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production.Bioengineering (Basel, Switzerland) · 2018Review
- Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic IsolateIranian journal of pharmaceutical research : IJPR · 2018Article
- Delignification and detoxification of peanut shell bio-waste using an extremely halophilic laccase from an Aquisalibacillus elongatus isolate.Extremophiles : life under extreme conditions · 2017Article
- Lipase catalysis in organic solvents: advantages and applications.Biological procedures online · 2016Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A haloalkaliphilic solvent-tolerant lipase was produced from Alkalibacillus salilacus within 48 h of growth in liquid medium. An overall 4.9-fold enhanced production was achieved over unoptimized media after medium optimization by statistical approaches. Plackett-Burman screening suggested lipase production maximally influenced by olive oil, KH2PO4, NaCl, and glucose; and response surface methodology predicted the appropriate levels of each parameter. Produced lipase was highly active and stable over broad ranges of temperature (15-65 °C), pH (4.0-11.0), and NaCl concentration (0-30 %) showing excellent thermostable, pH-stable, and halophilic properties. The enzyme was optimally active at pH 8.0 and 40 °C. Majority of cations, except some like Co(2+) and Al(3+) were positive signals for lipase activity. In addition, the presence of chemical agents and organic solvents with different log P ow was well tolerated by the enzyme. Finally, efficacy of lipase-mediated esterification of various alcohols with oleic acid in organic solvents was studied.
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