Evidence map›Paper›PMID 26197708›Full record

ArticleDiabetologia2015

Extremely rapid increase in fatty acid transport and intramyocellular lipid accumulation but markedly delayed insulin resistance after high fat feeding in rats.

Arend Bonen, Swati S Jain, Laelie A Snook, Xiao-Xia Han, Yuko Yoshida, Kathryn H Buddo, James S Lally, Elizabeth D Pask, Sabina Paglialunga, Marie-Soleil Beaudoin and 6 more

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Diabetologia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed, 1 pooled it
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
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  7. Article
  8. Intramuscular diacylglycerol accumulates with acute hyperinsulinemia in insulin-resistant phenotypes.American journal of physiology. Endocrinology and metabolism · 2024
    Article
  9. Review
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  12. The role of acetylation in obesity-induced cardiac metabolic alterations.Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques · 2024
    Review
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  17. Article
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  19. Article
  20. Effects of Lipid Overload on Heart in Metabolic Diseases.Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 3 countries.

Arend BonenDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1. arend.bonen@gmail.com.
Swati S JainDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Laelie A SnookDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Xiao-Xia HanDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Yuko YoshidaDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Kathryn H BuddoDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
James S LallyDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Elizabeth D PaskDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Sabina PaglialungaDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Marie-Soleil BeaudoinDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Jan F C GlatzDepartment of Molecular Genetics, Maastricht University, Maastricht, the Netherlands.
Joost J F P LuikenDepartment of Molecular Genetics, Maastricht University, Maastricht, the Netherlands.
Ewa HarasimDepartment of Physiology, Medical University of Bialystok, Bialystok, Poland.
David C WrightDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
Adrian ChabowskiDepartment of Physiology, Medical University of Bialystok, Bialystok, Poland.
Graham P HollowayDepartment of Human Health & Nutritional Sciences, University of Guelph, Guelph, ON, Canada, N1G 2W1.
University of Guelph · CAMaastricht University · NLMedical University of Białystok · PL

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

aims/hypothesisThe mechanisms for diet-induced intramyocellular lipid accumulation and its association with insulin resistance remain contentious. In a detailed time-course study in rats, we examined whether a high-fat diet increased intramyocellular lipid accumulation via alterations in fatty acid translocase (FAT/CD36)-mediated fatty acid transport, selected enzymes and/or fatty acid oxidation, and whether intramyocellular lipid accretion coincided with the onset of insulin resistance.

methodsWe measured, daily (on days 1-7) and/or weekly (for 6 weeks), the diet-induced changes in circulating substrates, insulin, sarcolemmal substrate transporters and transport, selected enzymes, intramyocellular lipids, mitochondrial fatty acid oxidation and basal and insulin-stimulated sarcolemmal GLUT4 and glucose transport. We also examined whether upregulating fatty acid oxidation improved glucose transport in insulin-resistant muscles. Finally, in Cd36-knockout mice, we examined the role of FAT/CD36 in intramyocellular lipid accumulation, insulin sensitivity and diet-induced glucose intolerance.

resultsWithin 2-3 days, diet-induced increases occurred in insulin, sarcolemmal FAT/CD36 (but not fatty acid binding protein [FABPpm] or fatty acid transporter [FATP]1 or 4), fatty acid transport and intramyocellular triacylglycerol, diacylglycerol and ceramide, independent of enzymatic changes or muscle fatty acid oxidation. Diet-induced increases in mitochondria and mitochondrial fatty acid oxidation and impairments in insulin-stimulated glucose transport and GLUT4 translocation occurred much later (≥21 days). FAT/CD36 ablation impaired insulin-stimulated fatty acid transport and lipid accumulation, improved insulin sensitivity and prevented diet-induced glucose intolerance. Increasing fatty acid oxidation in insulin-resistant muscles improved glucose transport. CONCLUSIONS/INTERPRETATIONS: High-fat feeding rapidly increases intramyocellular lipids (in 2-3 days) via insulin-mediated upregulation of sarcolemmal FAT/CD36 and fatty acid transport. The 16-19 day delay in the onset of insulin resistance suggests that additional mechanisms besides intramyocellular lipids contribute to this pathology.

Indexed as

AnimalsCD36 AntigensDiet, High-FatFatty Acid-Binding ProteinsFatty AcidsGlucoseGlucose Transporter Type 4Insulin ResistanceLipid MetabolismMaleMitochondriaMuscle CellsMuscle, SkeletalRatsRats, Sprague-DawleyCD36 AntigensFatty Acid-Binding ProteinsFatty AcidsGlucoseGlucose Transporter Type 4Slc2a4 protein, ratCeramideDiacylglycerolFatty acid oxidationGlucose transportGLUT4Insulin resistanceMitochondriaMusclePGC-1αTriacylglycerol

Identifiers

PMID26197708
OpenAlexW2142796533

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.