Evidence map›Paper›PMID 26197412›Full record

ArticleBiomaterials2015

Engineering an "infectious" T(reg) biomimetic through chemoselective tethering of TGF-β1 to PEG brush surfaces.

E Y Yang, J P Kronenfeld, K M Gattás-Asfura, A L Bayer, C L Stabler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 23 citations in OpenAlex.

  1. Review
  2. Review
  3. Immunometabolism of Liver Xenotransplantation and Prospective Solutions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  4. Unlocking Transplant Tolerance with Biomaterials.Advanced healthcare materials · 2025
    Review
  5. Article
  6. Review
  7. Clickable Biomaterials for Modulating Neuroinflammation.International journal of molecular sciences · 2022
    Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Review
  16. Novel technologies to engineer graft for tolerance induction.Current opinion in organ transplantation · 2016
    Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

E Y YangDiabetes Research Institute, University of Miami, Miami, FL, USA; Department of Biochemistry and Molecular Biology, University of Miami, Miami, FL, USA.
J P KronenfeldDiabetes Research Institute, University of Miami, Miami, FL, USA; Department of Medicine, University of Miami, Miami, FL, USA.
K M Gattás-AsfuraDiabetes Research Institute, University of Miami, Miami, FL, USA.
A L BayerDiabetes Research Institute, University of Miami, Miami, FL, USA; Department of Microbiology and Immunology, University of Miami, Miami, FL, USA.
C L StablerDiabetes Research Institute, University of Miami, Miami, FL, USA; Department of Biochemistry and Molecular Biology, University of Miami, Miami, FL, USA; Department of Biomedical Engineering, University of Miami, Miami, FL, USA; Department of Surgery, University of Miami, Miami, FL, USA. Electronic address: cstabler@bme.ufl.edu.
University of Miami · US

Funding

Functionalized, Nanoscale Coatings for Islet EncapsulationDP2DK083096 · NIDDK · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI STABLER, CHERIE L · 2008 to 2011
$2.5M
Engineering Ultrathin Immunomodulatory Coatings for Islet EncapsulationR01DK100654 · NIDDK · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI STABLER, CHERIE L · 2014 to 2018
$2.2M
NIDDK NIH HHS 1DP2 DK08309601NIDDK NIH HHS DP2 DK083096NIDDK NIH HHS R01 DK100654NIDDK NIH HHS R01DK100654
6 · The paper itself

Abstract

Modulation of immunological responses to allografts following transplantation is of pivotal importance to improving graft outcome and duration. Of the many approaches, harnessing the dominant tolerance induced by regulatory T cells (Treg) holds tremendous promise. Recent studies have highlighted the unique potency of cell surface-bound TGF-β1 on Treg for promoting infectious tolerance, i.e. to confer suppressive capacity from one cell to another. To mimic this characteristic, TGF-β1 was chemoselectively tethered to inert and viable polymer grafting platforms using Staudinger ligation. We report the synthesis and functional characterization of these engineered TGF-β1 surfaces. Inert beads tethered with TGF-β1 were capable of efficiently converting naïve CD4(+) CD62L(hi) T cells to functional Treg. Concordantly, translation of conjugation scheme from inert surfaces to viable cells also led to efficient generation of functional Treg. Further, the capacity of these platforms to generate antigen-specific Treg was demonstrated. These findings illustrate the unique faculty of tethered TGF-β1 biomaterial platforms to function as an "infectious" Treg and provide a compelling approach for generating tolerogenic microenvironments for allograft transplantation.

Indexed as

AnimalsAntigen-Presenting CellsBiomimetic MaterialsForkhead Transcription FactorsGreen Fluorescent ProteinsLymphocyte ActivationMaterials TestingMice, Inbred C57BLMice, TransgenicMicrospheresPhthalic AcidsPolyethylene GlycolsSurface PropertiesT-Lymphocytes, RegulatoryTransforming Growth Factor beta1Forkhead Transcription FactorsFoxp3 protein, mouseGreen Fluorescent ProteinsPhthalic AcidsPolyethylene Glycolsterephthalic acidTransforming Growth Factor beta1BiomaterialsImmunomodulationRegulatory T cellsStaudinger ligation

Identifiers

PMID26197412
PMCPMC4550500
OpenAlexW1499874826

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.