ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2015
Vinculin is required for cell polarization, migration, and extracellular matrix remodeling in 3D collagen.
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Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
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59 citing papers in PubMed, 115 citations in OpenAlex.
- Article
- Mechanometabolism of cell adhesion: Vinculin regulates bioenergetics via RhoA-ROCK.The Journal of cell biology · 2026Article
- Wnt5a Regulates Embryonic Müllerian Duct Development Through the Non-Canonical Wnt PCP Pathway.Cells · 2026Article
- Measurement of cellular traction forces during confined migration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanochemical waves in focal adhesions during cell migration.Science advances · 2025Article
- Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D.iScience · 2025Article
- GSK3 acts as a switch for transcriptional programs in a model of low-grade gliomagenesis.Acta neuropathologica communications · 2025Article
- Vinculin-Arp2/3 interaction inhibits branched actin assembly to control migration and proliferation.Life science alliance · 2025Article
- Comparison of vinculin tension in cellular monolayers and three-dimensional multicellular aggregates.Biomedical optics express · 2024Article
- B cell-based therapy produces antibodies that inhibit glioblastoma growth.The Journal of clinical investigation · 2024Article
- Dietary Polyphenols Effects on Focal Adhesion Plaques and Metalloproteinases in Cancer Invasiveness.Biomedicines · 2024Review
- Leader cells mechanically respond to aligned collagen architecture to direct collective migration.PloS one · 2024Article
- Confinement primes cells for faster migration by polarizing active mitochondria.Nanoscale advances · 2023Article
- Molecular basis and cellular functions of vinculin-actin directional catch bonding.Nature communications · 2023Article
- Vinculin is essential for sustaining normal levels of endogenous forces at cell-cell contacts.Biophysical journal · 2023Article
- Development of a Ratiometric Tension Sensor Exclusively Responding to Integrin Tension Magnitude in Live Cells.ACS sensors · 2023Article
- Cdk4 Regulates Glioblastoma Cell Invasion and Stemness and Is Target of a Notch Inhibitor Plus Resveratrol Combined Treatment.International journal of molecular sciences · 2023Article
- Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix.Biointerphases · 2023Article
- Vinculin transmits high-level integrin tensions that are dispensable for focal adhesion formation.Biophysical journal · 2023Article
- Elevated Serum Vinculin in Patients with HBV/HCV-Associated Liver Cirrhosis and Hepatocellular Carcinoma: A Pilot Study.Biologics : targets & therapy · 2023Article
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Authors and funding
13 authors at 7 institutions in 3 countries.
Ingo Thievessen*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA ithievessen@biomed.uni-erlangen.de.
Nikta Fakhri*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Julian Steinwachs*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Viola Kraus*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
R Scott McIsaac*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Liang Gao*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Bi-Chang Chen*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Michelle A Baird*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Michael W Davidson*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Eric Betzig*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Rudolf Oldenbourg*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Clare M Waterman*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Ben Fabry*Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA; Biophysics Group, Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany; Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA; Third Physics Institute-Biophysics, Georg-August-University, Göttingen, Germany; Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA; California Life Company, South San Francisco, California, USA; **Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; Department of Chemistry, Stony Brook University, Stony Brook, New York, USA; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA.
Friedrich-Alexander-Universität Erlangen-Nürnberg · DEMarine Biological Laboratory · USFlorida State University · USJanelia Research Campus · USNational Institutes of Health · USResearch Center for Applied Science, Academia Sinica · TWUniversity of Göttingen · DE
Funding
Mechanical Regulation of Cell AdhesionZIAHL005104 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI WATERMAN, CLARE MICHAL · 2009 to 2025
$30.9MIntegration of Actin Dynamics and Adhesion in Cell MigrationZIAHL005105 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI WATERMAN, CLARE MICHAL · 2009 to 2025
$16.5MDevelopment of New Automated Polarized Light MicroscopeR01EB002583 · NIBIB · MARINE BIOLOGICAL LABORATORY · PI OLDENBOURG, RUDOLF · 2004 to 2012
$4.4MPhysiology: Modern Cell Biology Using Microscopic, Biochemical and Computational ApproachesR13GM085967 · NIGMS · MARINE BIOLOGICAL LABORATORY · PI FLETCHER, DANIEL A, HYMAN, LINDA E · 2008 to 2019
$1.7MIntramural NIH HHSNIBIB NIH HHS R01 EB002583NIGMS NIH HHS R13 GM085967
Abstract
Vinculin is filamentous (F)-actin-binding protein enriched in integrin-based adhesions to the extracellular matrix (ECM). Whereas studies in 2-dimensional (2D) tissue culture models have suggested that vinculin negatively regulates cell migration by promoting cytoskeleton-ECM coupling to strengthen and stabilize adhesions, its role in regulating cell migration in more physiologic, 3-dimensional (3D) environments is unclear. To address the role of vinculin in 3D cell migration, we analyzed the morphodynamics, migration, and ECM remodeling of primary murine embryonic fibroblasts (MEFs) with cre/loxP-mediated vinculin gene disruption in 3D collagen I cultures. We found that vinculin promoted 3D cell migration by increasing directional persistence. Vinculin was necessary for persistent cell protrusion, cell elongation, and stable cell orientation in 3D collagen, but was dispensable for lamellipodia formation, suggesting that vinculin-mediated cell adhesion to the ECM is needed to convert actin-based cell protrusion into persistent cell shape change and migration. Consistent with this finding, vinculin was necessary for efficient traction force generation in 3D collagen without affecting myosin II activity and promoted 3D collagen fiber alignment and macroscopical gel contraction. Our results suggest that vinculin promotes directionally persistent cell migration and tension-dependent ECM remodeling in complex 3D environments by increasing cell-ECM adhesion and traction force generation.
Indexed as
AnimalsCell MovementCell PolarityCollagenExtracellular MatrixFibroblastsMiceMice, KnockoutMyosin Type IIPseudopodiaVinculinCollagenMyosin Type IIVinculin3D cell migrationcell morphodynamicsintegrintraction force generation
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