ArticleScientific reports2015
Nonfunctional ingestion of plant miRNAs in silkworm revealed by digital droplet PCR and transcriptome analysis.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Review
- Cross-kingdom regulation of plant microRNAs: potential application in crop improvement and human disease therapeutics.Frontiers in plant science · 2024Review
- Cross-Kingdom Regulation of Plant-Derived miRNAs in Modulating Insect Development.International journal of molecular sciences · 2023Review
- Genome-wide identification and validation of tomato-encoded sRNA as the cross-species antifungal factors targeting the virulence genes ofFrontiers in plant science · 2023Article
- Identification and profiling of stable microRNAs in hemolymph of young and oldCurrent research in insect science · 2022Article
- Overexpression of an Osa-miR162a Derivative in Rice Confers Cross-Kingdom RNA Interference-Mediated Brown Planthopper Resistance without Perturbing Host Development.International journal of molecular sciences · 2021Article
- RNAs on the Go: Extracellular Transfer in Insects with Promising Prospects for Pest Management.Plants (Basel, Switzerland) · 2021Review
- Small RNAs in eucaryotes: new clues for amplifying microRNA benefits.Cell & bioscience · 2020Review
- Plant miRNA Cross-Kingdom Transfer Targeting Parasitic and Mutualistic Organisms as a Tool to Advance Modern Agriculture.Frontiers in plant science · 2020Review
- Host Plant-Derived miRNAs Potentially Modulate the Development of a Cosmopolitan Insect Pest,Biomolecules · 2019Article
- Review
- Prediction of plant-derived xenomiRs from plant miRNA sequences using random forest and one-dimensional convolutional neural network models.BMC genomics · 2018Article
- miRNA-Mediated Interactions in and between Plants and Insects.International journal of molecular sciences · 2018Review
- Evidence for plant-derived xenomiRs based on a large-scale analysis of public small RNA sequencing data from human samples.PloS one · 2018Article
- Detection of dietetically absorbed maize-derived microRNAs in pigs.Scientific reports · 2017Article
- New Insight into Inter-kingdom Communication: Horizontal Transfer of Mobile Small RNAs.Frontiers in microbiology · 2017Review
- Horizontal Transfer of Small RNAs to and from Plants.Frontiers in plant science · 2015Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since a plant miRNA (miR168) cross-regulating a mammalian transcript was reported, miRNA-mediated cross-kingdom communication has become one of the most compelling but controversial topics. In the present study, we used silkworm and mulberry, which is a model for studies on the interactions between the insect and its host plant, to address whether miRNA-mediated cross-kingdom communication is a common phenomenon. The results of TA clone, Sanger sequencing and droplet digital PCR demonstrated that several mulberry-derived miRNAs could enter to silkworm hemolymph and multiple tested tissues. Synthetic miR166b was also detected in hemolymph and fat body. However, the ingestion of synthetic miR166b did not play roles in silkworm physiological progress, which was revealed by RNA-seq analyses, RT-PCR, and phenotypic investigations. Mulberry miRNAs are convincingly transferred to the silkworm orally and no physiological process associated with the miRNAs was demonstrable. The results provided a new aspect of cross-kingdom miRNA transfer.
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Registered trials
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