ArticleBMC veterinary research2015
First isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzania.
Article in BMC veterinary research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Brucellosis in wildlife in Africa: a systematic review and meta-analysis.Scientific reports · 2021Pooled it
- Prevalence and speciation of brucellosis in febrile patients from a pastoralist community of Tanzania.Scientific reports · 2020Trial
- Seroprevalence, associated risk factors, and molecular detection of Brucella spp. in cattle, sheep, goats and humans in Mvomero district, Morogoro, Tanzania.One health outlook · 2026Article
- First report of the isolation, characterization, and genotyping of Brucella abortus biovar 9 in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Comparative Evaluation of Diagnostic Tests for Brucellosis in Humans and Animals: A Meta-Analytical Approach.Veterinary sciences · 2025Article
- Seroprevalence of Brucellosis in Haryana, India: A Study Using Rose Bengal Plate Test and Enzyme-Linked Immunosorbent Assay.Pathogens (Basel, Switzerland) · 2025Article
- Livestock production losses attributable to brucellosis in northern and central Tanzania: Application of an epidemiological-economic modelling framework.PLoS neglected tropical diseases · 2025Article
- Article
- Complete genome sequence ofMicrobiology resource announcements · 2024Article
- Characterization ofVeterinary research forum : an international quarterly journal · 2024Article
- Scent detection of Brucella abortus by African giant pouched rats (Cricetomys ansorgei).BMC veterinary research · 2023Article
- Evaluation of serological diagnostic tests for bovine brucellosis in dairy cattle herds in an endemic area: a multicenter study.Tropical animal health and production · 2023Article
- The Status and Risk Factors of Brucellosis in Smallholder Dairy Cattle in Selected Regions of Tanzania.Veterinary sciences · 2023Article
- Original and introduced lineages co-driving the persistence ofFrontiers in public health · 2023Article
- Seroprevalence of brucellosis and molecular characterization of Brucella spp. from slaughtered cattle in Rwanda.PloS one · 2022Article
- Circulating Brucella species in wild animals of the Serengeti ecosystem, Tanzania.One health outlook · 2021Article
- Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania.PLoS neglected tropical diseases · 2021Article
- Brucellosis: Prevalence with reference to East African community countries - A rapid review.Veterinary medicine and science · 2021Review
- Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya.Scientific reports · 2021Article
- Brucellosis in Camels and Humans: Seroprevalence and Associated Risk Factors in Amibara District of Afar Region, Ethiopia.Veterinary medicine international · 2021Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBrucellosis is a disease of worldwide public health and economic importance. Successful control is based on knowledge of epidemiology and strains present in an area. In developing countries, most investigations are based on serological assays. This study aimed at investigating a dairy herd experiencing abortions in order to establish within-herd seroprevalence to Brucella spp., identify, characterize Brucella strains by Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA-VNTR) and investigate possible spillover to other species.
resultsThe within-herd seroprevalence in cattle (n = 200) was 48 % (95 % CI 41-55), using an indirect ELISA, while the Rose Bengal Test (RBT) yielded lower prevalence (21.5 %; 95 % CI 16-27). Two sheep (n = 35) and one goat (n = 50) were seropositive using ELISA while none of the dogs (n = 6) was positive with the RBT. Three Brucella were isolated from an aborted fetus and associated membranes. Real time PCR (IS711), Bruce-ladder and classical biotyping classified the isolates as B. abortus biovar 3. MLVA-VNTR revealed two different but closely related genotypes. The isolates showed unique profiles, providing the first genotypic data from Tanzania. These genotypes were not related to B. abortus biovar 3 reference strain Tulya originally isolated from a human patient in Uganda in 1958, unlike the genotypes isolated and characterized recently in Kenya. High within-herd prevalence, isolation of the pathogen and abortion confirm that B. abortus is circulating in this herd with cattle as reservoir hosts. A low seroprevalence in sheep and goats suggests a spillover of B. abortus from cattle to small ruminants in the herd.
conclusionsThis is the first isolation and characterization of B. abortus biovar 3 from a dairy cow with abortion in Tanzania. The origin of the Tanzanian genotypes remain elusive, although they seem to be related to genotypes found in Europe, Turkey and China but not related to B. abortus biovar 3 reference strain or genotypes from Kenya. Importantly, replacement heifers are commonly sourced from large farms like this to smallholder farmers, which poses risk of spread of bacteria to other herds. B. abortus is a significant zoonotic risk and animal health problem in this production system, therefore further studies on humans is recommended.
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