ArticleScientific reports2015
An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
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- Yeast MoClo Secretion and Surface Display Toolkit 2.0: Improvements and Applications for Analysis of protein-protein Interactions and Whole-Cell Biocatalysis.ACS synthetic biology · 2026Article
- Biological parts in yeast synthetic biology: from regulatory elements to predictive design platforms.FEMS yeast research · 2026Review
- A yeast surface display platform for screening of non-enzymatic protein secretion in Kluyveromyces lactis.Applied microbiology and biotechnology · 2024Article
- Secretory Production of theJournal of microbiology and biotechnology · 2024Article
- A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization of Heterologous Protein Secretion and Surface Display inACS synthetic biology · 2024Article
- EngineeringEngineering microbiology · 2024Review
- Efficient Escorting Strategy for Aggregation-Prone Notch EGF Repeats with Sparcl1.Molecules (Basel, Switzerland) · 2024Article
- Article
- Modulation of Kex2p Cleavage Site for In Vitro Processing of Recombinant Proteins Produced byJournal of microbiology and biotechnology · 2023Article
- Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach.Microbial cell factories · 2023Article
- An outlook to sophisticated technologies and novel developments for metabolic regulation in theFrontiers in bioengineering and biotechnology · 2023Review
- Fructan Biosynthesis by Yeast Cell Factories.Journal of microbiology and biotechnology · 2022Review
- Production of autolysis-proof Kex2 protease from Candida albicans in Saccharomyces cerevisiae for in vitro processing of fusion proteins.Applied microbiology and biotechnology · 2022Article
- Tailoring in fungi for next generation cellulase production with special reference to CRISPR/CAS system.Systems microbiology and biomanufacturing · 2022Review
- Secretome-based screening of fusion partners and their application in recombinant protein secretion in Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2022Article
- Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification.Frontiers in microbiology · 2020Review
- Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts.Scientific reports · 2019Article
- Efficient production of levan using a recombinant yeast Saccharomyces cerevisiae hypersecreting a bacterial levansucrase.Journal of industrial microbiology & biotechnology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion partner. A TFP library was constructed from a genomic and truncated cDNA library by using the invertase-based signal sequence trap technique. The efficiency of the system was demonstrated using two rarely secreted proteins, human interleukin (hIL)-2 and hIL-32. Optimal TFPs for secretion of hIL-2 and hIL-32 were easily selected, yielding secretion of these proteins up to hundreds of mg/L. Moreover, numerous uncovered yeast secretion signals and fusion partners were identified, leading to efficient secretion of various recombinant proteins. Selected TFPs were found to be useful for the hypersecretion of other recombinant proteins at yields of up to several g/L. This screening technique could provide new methods for the production of various types of difficult-to-express proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.