ArticleScientific reports2015
The pain receptor TRPV1 displays agonist-dependent activation stoichiometry.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Distinct functional open states of the polymodal nociceptor TRPV1.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The psychoactive cannabinoid THC inhibits peripheral nociceptors by targeting NaNeuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Article
- How much does TRPV1 deviate from an ideal MWC-type protein?Biophysical journal · 2024Article
- TRPM5 activation depends on a synergistic effect of calcium and PKC phosphorylation.Communications biology · 2024Article
- A pentameric TRPV3 channel with a dilated pore.Nature · 2023Article
- Cloxyquin activates hTRESK by allosteric modulation of the selectivity filter.Communications biology · 2023Article
- Opening of capsaicin receptor TRPV1 is stabilized equally by its four subunits.The Journal of biological chemistry · 2023Article
- A molecular perspective on identifying TRPV1 thermosensitive regions and disentangling polymodal activation.Temperature (Austin, Tex.) · 2023Review
- Venom Peptide Toxins Targeting the Outer Pore Region of Transient Receptor Potential Vanilloid 1 in Pain: Implications for Analgesic Drug Development.International journal of molecular sciences · 2022Review
- Article
- Imaging with Ion Channels.Analytical chemistry · 2021Article
- Evidence that the TRPV1 S1-S4 membrane domain contributes to thermosensing.Nature communications · 2020Article
- Properties of Thermal Analgesia in a Human Chronic Low Back Pain Model.Journal of pain research · 2020Article
- The Mysteries of Capsaicin-Sensitive Afferents.Frontiers in physiology · 2020Review
- Endogenous and Exogenous Vanilloids Evoke Disparate TRPV1 Activation to Produce Distinct Neuronal Responses.Frontiers in pharmacology · 2020Article
- Transient Photoinactivation of Cell Membrane Protein Activity without Genetic Modification by Molecular Hyperthermia.ACS nano · 2019Article
- TRPV1 mediates astrocyte activation and interleukin-1β release induced by hypoxic ischemia (HI).Journal of neuroinflammation · 2019Article
- Two Vanilloid Ligand Bindings Per Channel Are Required to Transduce Capsaicin-Activating Stimuli.Frontiers in molecular neuroscience · 2019Article
- TRPV1 pore turret dictates distinct DkTx and capsaicin gating.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Activation Stoichiometry and Pore Architecture of TRPA1 Probed with Channel Concatemers.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The receptor channel TRPV1 (Transient Receptor Potential Vanilloid 1) is expressed by primary afferent sensory neurons of the pain pathway, where it functions as a sensor of noxious heat and various chemicals, including eicosanoids, capsaicin, protons and peptide toxins. Comprised of four identical subunits that organize into a non-selective cationic permeable channel, this receptor has a variety of binding sites responsible for detecting their respective agonists. Although its physiological role as a chemosensor has been described in detail, the stoichiometry of TRPV1 activation by its different ligands remains unknown. Here, we combined the use of concatemeric constructs harboring mutated binding sites with patch-clamp recordings in order to determine the stoichiometry for TRPV1 activation through the vanilloid binding site and the outer-pore domain by capsaicin and protons, respectively. We show that, while a single capsaicin-bound subunit was sufficient to achieve a maximal open-channel lifetime, all four proton-binding sites were required. Thus, our results demonstrate a distinct stoichiometry of TRPV1 activation through two of its different agonist-binding domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.