Evidence map›Paper›PMID 26194790›Full record

ArticleStem cell research & therapy2015

Development of a novel imaging system for cell therapy in the brain.

Maria-Adelaide Micci, Debbie R Boone, Margaret A Parsley, Jingna Wei, Igor Patrikeev, Massoud Motamedi, Helen L Hellmich

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 10 citations in OpenAlex.

  1. Reporter Genes for Brain Imaging Using MRI, SPECT and PET.International journal of molecular sciences · 2022
    Review
  2. Molecular therapy. Methods & clinical development · 2020
    Article
  3. New imaging probes to track cell fate: reporter genes in stem cell research.Cellular and molecular life sciences : CMLS · 2017
    Review
  4. Review
  5. Review
  6. Article
  7. Article
  8. Molecular imaging for stem cell therapy in the brain.Stem cell research & therapy · 2015
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Maria-Adelaide MicciDepartment of Anesthesiology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX, 77555, USA. mmicci@utmb.edu.
Debbie R BooneDepartment of Anesthesiology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX, 77555, USA. drkenned@utmb.edu.
Margaret A ParsleyDepartment of Anesthesiology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX, 77555, USA. maparsle@utmb.edu.
Jingna WeiCenter for Biomedical Engineering, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555, USA. jiwei@utmb.edu.
Igor PatrikeevCenter for Biomedical Engineering, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555, USA. igpatrik@utmb.edu.
Massoud MotamediCenter for Biomedical Engineering, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555, USA. mmotamed@utmb.edu.
Helen L HellmichDepartment of Anesthesiology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX, 77555, USA. hhellmic@utmb.edu.
The University of Texas Medical Branch at Galveston · US

Funding

UTMB CLINICAL AND TRANSLATIONAL SCIENCE AWARD: NASA BED REST STUDIESUL1RR029876 · NCRR · UNIVERSITY OF TEXAS MED BR GALVESTON · PI BRASIER, ALLAN R. · 2009 to 2011
$17.7M
NCRR NIH HHS 1UL1RR029876-01NCRR NIH HHS UL1 RR029876
6 · The paper itself

Abstract

introductionStem cells have been evaluated as a potential therapeutic approach for several neurological disorders of the central and peripheral nervous system as well as for traumatic brain and spinal cord injury. Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site of implantation and track the migration of stem cells in vivo hampers the development of stem cell therapy and its clinical application. In this report, we present data that demonstrate the feasibility of using the human sodium iodide symporter (hNIS) as a reporter gene for tracking neural stem cells (NSCs) after transplantation in the brain by using single-photon emission tomography/computed tomography (SPECT/CT) imaging.

methodsNSCs were isolated from the hippocampus of adult rats (Hipp-NSCs) and transduced with a lentiviral vector containing the hNIS gene. Hipp-NSCs expressing the hNIS (NIS-Hipp-NSCs) were characterized in vitro and in vivo after transplantation in the rat brain and imaged by using technetium-99m ((99m)Tc) and a small rodent SPECT/CT apparatus. Comparisons were made between Hipp-NSCs and NIS-Hipp-NSCs, and statistical analysis was performed by using two-tailed Student's t test.

resultsOur results show that the expression of the hNIS allows the repeated visualization of NSCs in vivo in the brain by using SPECT/CT imaging and does not affect the ability of Hipp-NSCs to generate neuronal and glial cells in vitro and in vivo.

conclusionsThese data support the use of the hNIS as a reporter gene for non-invasive imaging of NSCs in the brain. The repeated, non-invasive tracking of implanted cells will accelerate the development of effective stem cell therapies for traumatic brain injury and other types of central nervous system injury.

Indexed as

AnimalsBlotting, WesternBrainCell- and Tissue-Based TherapyCell ProliferationCells, CulturedDiagnostic ImagingHippocampusMaleNeural Stem CellsRatsRats, Sprague-DawleyTomography, Emission-Computed, Single-Photon

Identifiers

PMID26194790
PMCPMC4534109
OpenAlexW1907445408

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.