ArticleBMC plant biology2015
Development of transgenic wheat (Triticum aestivum L.) expressing avidin gene conferring resistance to stored product insects.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 32 citations in OpenAlex.
- Acceptance and utilization efficiency of a purple durum wheat genotype by Sitophilus granarius (L.).Scientific reports · 2023Article
- Domestication of newly evolved hexaploid wheat-A journey of wild grass to cultivated wheat.Frontiers in genetics · 2022Review
- Overexpression of Soybean-Derived Lunasin in Wheat and Assessment of Its Anti-Proliferative Activity in Colorectal Cancer HT-29 Cells.International journal of molecular sciences · 2020Article
- Characterization, cloning, expression and bioassay ofSaudi journal of biological sciences · 2020Article
- Article
- Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize.Plant methods · 2017Article
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Corrections and comments
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Authors and funding
16 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWheat is considered the most important cereal crop all over the world. The wheat weevil Sitophilus granarius is a serious insect pests in much of the wheat growing area worldwide and is responsible for significant loss of yield. Avidin proteins has been proposed to function as plant defense agents against insect pests.
resultsA synthetic avidin gene was introduced into spring wheat (Triticum aestivum L.) cv. Giza 168 using a biolistic bombardment protocol. The presence and expression of the transgene in six selected T0 transgenic wheat lines were confirmed at the molecular level. Accumulation of avidin protein was detected in transgenic plants compared to non-transgenic plants. Avidin transgene was stably integrated, transcribed and translated as indicated by Southern blot, ELISA, and dot blot analyses, with a high level of expression in transgenic wheat seeds. However, no expression was detected in untransformed wheat seeds. Functional integrity of avidin was confirmed by insect bioassay. The results of bioassay using transgenic wheat plants challenged with wheat weevil revealed 100 % mortality of the insects reared on transgenic plants after 21 days.
conclusionTransgenic wheat plants had improved resistance to Sitophilus granarius.
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Registered trials
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