ArticleBMC genomics2015
Evolutionary engineering of a wine yeast strain revealed a key role of inositol and mannoprotein metabolism during low-temperature fermentation.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Genomic tales of wine yeasts: from domestication to functional innovation.FEMS yeast research · 2026Review
- Response of alcohol fermentation strains, mixed fermentation and extremozymes interactions on wine flavor.Frontiers in microbiology · 2025Review
- Different Nitrogen Consumption Patterns in Low Temperature Fermentations in the Wine YeastFoods (Basel, Switzerland) · 2024Article
- Experimental Evolution in a Warming World: The Omics Era.Molecular biology and evolution · 2024Review
- Transcriptomic and metabolomic correlation analysis: effect of initial SOFrontiers in microbiology · 2024Article
- Contributions of Adaptive Laboratory Evolution towards the Enhancement of the Biotechnological Potential of Non-Conventional Yeast Species.Journal of fungi (Basel, Switzerland) · 2023Review
- Adaptation in Outbred Sexual Yeast is Repeatable, Polygenic and Favors Rare Haplotypes.Molecular biology and evolution · 2022Article
- Directed evolution as an approach to increase fructose utilization in synthetic grape juice by wine yeast AWRI 796.FEMS yeast research · 2022Article
- The Plasma Membrane at the Cornerstone Between Flexibility and Adaptability: Implications forFrontiers in microbiology · 2021Review
- Next Generation Winemakers: Genetic Engineering inBioengineering (Basel, Switzerland) · 2020Review
- Enhanced Wort Fermentation withApplied and environmental microbiology · 2018Article
- Specificity of genome evolution in experimental populations ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Yeast's balancing act between ethanol and glycerol production in low-alcohol wines.Microbial biotechnology · 2017Review
- Correlation between Low Temperature Adaptation and Oxidative Stress in Saccharomyces cerevisiae.Frontiers in microbiology · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundWine produced at low temperature is often considered to improve sensory qualities. However, there are certain drawbacks to low temperature fermentations: e.g. low growth rate, long lag phase, and sluggish or stuck fermentations. Selection and development of new Saccharomyces cerevisiae strains well adapted at low temperature is interesting for future biotechnological applications. This study aimed to select and develop wine yeast strains that well adapt to ferment at low temperature through evolutionary engineering, and to decipher the process underlying the obtained phenotypes.
resultsWe used a pool of 27 commercial yeast strains and set up batch serial dilution experiments to mimic wine fermentation conditions at 12 °C. Evolutionary engineering was accomplished by using the natural yeast mutation rate and mutagenesis procedures. One strain (P5) outcompeted the others under both experimental conditions and was able to impose after 200 generations. The evolved strains showed improved growth and low-temperature fermentation performance compared to the ancestral strain. This improvement was acquired only under inositol limitation. The transcriptomic comparison between the evolved and parental strains showed the greatest up-regulation in four mannoprotein coding genes, which belong to the DAN/TIR family (DAN1, TIR1, TIR4 and TIR3). Genome sequencing of the evolved strain revealed the presence of a SNP in the GAA1 gene and the construction of a site-directed mutant (GAA1 (Thr108)) in a derivative haploid of the ancestral strain resulted in improved fermentation performance. GAA1 encodes a GPI transamidase complex subunit that adds GPI, which is required for inositol synthesis, to newly synthesized proteins, including mannoproteins.
conclusionsIn this study we demonstrate the importance of inositol and mannoproteins in yeast adaptation at low temperature and the central role of the GAA1 gene by linking both metabolisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.