Evidence map›Paper›PMID 26194162›Full record

ArticleMolecular genetics and metabolism2015

The mouse wellhaarig (we) mutations result from defects in epidermal-type transglutaminase 3 (Tgm3).

Brett M Brennan, Minh T Huynh, Mohammed A Rabah, Hailie E Shaw, Jason J Bisaillon, Legairre A Radden, Tu V Nguyen, Thomas R King

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In one paragraph

Article in Molecular genetics and metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

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6citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Brett M BrennanBiomolecular Sciences, Central Connecticut State University, 1615 Stanley Street, New Britain, CT 06053, USA.
Minh T HuynhBiomolecular Sciences, Central Connecticut State University, 1615 Stanley Street, New Britain, CT 06053, USA.
Mohammed A RabahBiomolecular Sciences, Central Connecticut State University, 1615 Stanley Street, New Britain, CT 06053, USA.
Hailie E ShawBiomolecular Sciences, Central Connecticut State University, 1615 Stanley Street, New Britain, CT 06053, USA.
Jason J BisaillonBiomolecular Sciences, Central Connecticut State University, 1615 Stanley Street, New Britain, CT 06053, USA.
Legairre A RaddenBiomolecular Sciences, Central Connecticut State University, 1615 Stanley Street, New Britain, CT 06053, USA.
Tu V NguyenBiomolecular Sciences, Central Connecticut State University, 1615 Stanley Street, New Britain, CT 06053, USA.
Thomas R KingBiomolecular Sciences, Central Connecticut State University, 1615 Stanley Street, New Britain, CT 06053, USA. Electronic address: kingt@ccsu.edu.

Funding

Positional cloning of mutations that disrupt hair development in mice.R15AR059572 · NIAMS · CENTRAL CONNECTICUT STATE UNIVERSITY · PI KING, THOMAS R · 2011 to 2011
$288k
NIAMS NIH HHS R15 AR059572NIAMS NIH HHS R15-AR059572
6 · The paper itself

Abstract

The recessive wellhaarig (we) mutations, named for the wavy coat and curly whiskers they generate in homozygotes, have previously been mapped on mouse Chromosome 2. To further limit the possible location of the we locus, we crossed hybrid (C57BL/6×AKR)F1, we(4J)/+ females with AKR, we(4J)/we(4J) mutant males to create a large backcross family that was typed for various microsatellite markers and single-nucleotide polymorphisms (SNPs) that distinguish strains AKR and B6. This analysis restricted the location of we(4J) between sites that flank only one gene known to be expressed in skin: epidermal-type transglutaminase 3 (Tgm3). To test Tgm3 as a candidate for the basis of the wellhaarig phenotype we took two approaches. First, we sequenced all Tgm3 coding regions in mice homozygous for four independent, naturally-occurring wellhaarig alleles (we, we(Bkr), we(3J) and we(4J)) and found distinct defects in three of these mutants. Second, we crossed mice homozygous for an induced mutant allele of Tgm3 (Tgm3(Btlr)) with mice heterozygous for one of the wellhaarig alleles we possess (we(4J) or we(Bkr)) to test for complementation. Because the progeny inheriting both a recessive we allele and a recessive Tgm3(Btlr) allele displayed wavy hair, we conclude that the classic wellhaarig mutations result from defects in Tgm3.

Indexed as

MutationAllelesAnimalsChromosome MappingEpidermisFemaleGenetic Complementation TestHairHeterozygoteHomozygoteMaleMice, Inbred AKRMice, Inbred C57BLMicrosatellite RepeatsPhenotypePolymorphism, Single NucleotideTgm3 protein, mouseTransglutaminasesComplementation testingHair morphologyIntraspecific backcross mappingPositional candidate approach

Identifiers

PMID26194162
PMCPMC4640993

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