ArticleGenome biology2015
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Article in Genome biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 542 papers.
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542 citing papers in PubMed.
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- Mutual antagonism between auxin and diacylglycerol acyltransferase 2 regulates high-temperature-induced hypocotyl elongation.The New phytologist · 2026Article
- Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants.The New phytologist · 2026Article
- Assessing the relative contributions of different Arabidopsis thaliana stigma factors to pollen hydration.Plant reproduction · 2026Article
- The sperm cell-surface protein GEX3 coordinates with GEX2 and DMP8/9 to mediate sperm-egg adhesion and fusion in Arabidopsis.Plant communications · 2026Article
- GEX3 interacts with GEX2 to function in gamete attachment and plasma membrane fusion in Arabidopsis.Plant communications · 2026Article
- A MAP-SNARE interaction links cortical microtubules to the exocytic fusion machinery during auxin-dependent growth remodeling.Science advances · 2026Article
- Systemic and Local Regulation of Root Growth by Vascular Trehalose 6-Phosphate is Correlated With Re-allocation of Primary Metabolites Between Shoots and Roots.Plant, cell & environment · 2026Article
- Recent advancements in improving cross-species applicability of bioengineered AAV capsids.Gene therapy · 2026Review
- LURP1, a SARD1-regulated protein, coordinates with the helper NLR ADR1 to promote plant immunity.The New phytologist · 2026Article
- A redox-regulated RCC1-like protein controls catalase activity in Arabidopsis.Nature communications · 2026Article
- Dual Cytoplasmic and Chloroplastic Mechanisms Fine-Tune Chloroplast Division through ARC3 Protein Stability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A VIM2/4 deletion, and premature truncations of CMT2 and FBX5, drive DNA methylation changes after colonization of a novel habitat.Nature plants · 2026Article
- The feedback regulation between DREB2C and E3 ligases DEL1/DEL2 for balancing the initial growth and ABA signaling in Arabidopsis.The New phytologist · 2026Article
- EARLY ABORTION 1 is an evolutionarily conserved gene required for plant reproduction.Journal of experimental botany · 2026Article
482 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome editing system are usually mosaics in the T1 generation. In this study, we used egg cell-specific promoters to drive the expression of Cas9 and obtained non-mosaic T1 mutants for multiple target genes with high efficiency. Comparisons of 12 combinations of eight promoters and two terminators found that the efficiency of the egg cell-specific promoter-controlled CRISPR/Cas9 system depended on the presence of a suitable terminator, and the composite promoter generated by fusing two egg cell-specific promoters resulted in much higher efficiency of mutation in the T1 generation compared with the single promoters.
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