ArticleBiotechnology progress
Correlating intracellular nonviral polyplex localization with transfection efficiency using high-content screening.
Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
4 citing papers in PubMed.
- Sequence-defined polymers for predictable gene delivery.Nature reviews. Chemistry · 2026Review
- The Spatial Distribution of Lipophilic Cations in Gradient Copolymers Regulates Polymer-pDNA Complexation, Polyplex Aggregation, and Intracellular pDNA Delivery.Biomacromolecules · 2024Article
- Nanoparticle-Based Delivery of CRISPR/Cas9 Genome-Editing Therapeutics.The AAPS journal · 2018Review
- A simple, rapid method for evaluation of transfection efficiency based on fluorescent dye.Bioengineered · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
High-content screening (HCS) has gained interest in cellular imaging because of its ability to provide statistically significant data from multiple parameters simultaneously in cell-based assays. Although HCS has been mainly used in drug discovery, it has other potentially useful applications, such as elucidating the processes involved in nonviral gene vector-mediated gene delivery, as was explored in this study. HCS was used to measure transfection efficiency and cytotoxicities of polyplexes made from fluorescently labeled polyethylenimine (PEI) and pDNA encoding EGFP (pEGFP-N1). The results generated using HCS were confirmed using more conventional and labor-intensive methods. For the first time, a relationship between transfected cells and the number of polyplexes in the cytoplasm was shown. Four to five polyplex signals were found in the cytoplasm of successfully transfected cells, whilst nontransfected cells harbored less than one polyplex signal within the cytoplasm. HCS has the potential to be used as a tool in the field of gene delivery. HCS can not only simultaneously measure transfection efficiency and cytotoxicity of various nonviral gene vectors; it can also be used to track such vectors through various subcellular compartments.
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Registered trials
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