ArticleEuropean journal of oral sciences2015
Hypoxia increases the expression of enamel genes and cytokines in an ameloblast-derived cell line.
Article in European journal of oral sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.
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Who cites it
8 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.
- An update of the aetiological factors involved in molar incisor hypomineralisation (MIH): a systematic review and meta-analysis.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2022Pooled it
- Presumptive Bilirubin-Related Chlorodontia and Developmental Enamel Defects of the Primary Dentition in an Extremely Preterm Infant: A Case Report.Journal of clinical medicine · 2026Article
- High prevalence of molar-incisor hypomineralisation in sickle cell disease children and the association with haematologic profile: a retrospective cohort study.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2026Article
- Expression ofInternational journal of molecular sciences · 2025Article
- Correlation of the Apgar Score with Dental Caries in 3- to 5-year-old Iranian Children.Medical journal of the Islamic Republic of Iran · 2024Article
- Hard Dental Tissues Regeneration-Approaches and Challenges.Materials (Basel, Switzerland) · 2021Review
- Enamel biomimetics-fiction or future of dentistry.International journal of oral science · 2019Review
- Five-minute Apgar score ≤ 5 and Molar Incisor Hypomineralisation (MIH) - a case control study.BMC oral health · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The aim of this study was to investigate the effect of hypoxic conditions on the expression of enamel genes and on the secretion of alkaline phosphatase (ALP), lactate dehydrogenase (LDH), cytokines, and interleukins by an ameloblast-derived cell line. Murine ameloblast-derived cells (LS-8 cells) were exposed to 1% oxygen for 24 and 48 h and harvested after 1, 2, 3, and 7 d. The effect of culture in hypoxic conditions on the expression of structural enamel matrix genes and on the secretion of cytokines and interleukins, as well as ALP and LDH, into the cell-culture medium was calculated relative to the expression and secretion of these factors by untreated cells (controls) at each time point. Hypoxia increased expression of the structural enamel matrix genes amelogenin (Amelx), ameloblastin (Ambn), and enamelin (Enam), and the enamel protease matrix metalloproteinase-20 (Mmp20). Expression of hypoxia-inducible factor 1-alpha (Hif1α), and secretion of several vascularization factors and pro-inflammatory factors, were increased after 24 and 48 h of hypoxia. The ALP activity was reduced after 24 and 48 h of hypoxia, whereas the LDH level in the cell-culture medium was higher after 24 h of hypoxic conditions compared with 48 h. In conclusion, hypoxic exposure may disrupt the controlled fine-tuned expression and processing of enamel genes, and promote the secretion of pro-inflammatory factors.
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Registered trials
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