Evidence map›Paper›PMID 26193423›Full record

ArticleFEBS letters2015

Recognition of xyloglucan by the crystalline cellulose-binding site of a family 3a carbohydrate-binding module.

Mercedes C Hernandez-Gomez, Maja G Rydahl, Artur Rogowski, Carl Morland, Alan Cartmell, Lucy Crouch, Aurore Labourel, Carlos M G A Fontes, William G T Willats, Harry J Gilbert and 1 more

Abstract read
In one paragraph

Article in FEBS letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mercedes C Hernandez-GomezCentre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
Maja G RydahlDepartment of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
Artur RogowskiInstitute for Cell and Molecular Biosciences, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Carl MorlandInstitute for Cell and Molecular Biosciences, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Alan CartmellInstitute for Cell and Molecular Biosciences, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Lucy CrouchInstitute for Cell and Molecular Biosciences, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Aurore LabourelInstitute for Cell and Molecular Biosciences, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Carlos M G A FontesCIISA - Faculdade de Medicina Veterinária, Universidade de Lisboa, Pólo Universitário do Alto da Ajuda, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.
William G T WillatsDepartment of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
Harry J GilbertInstitute for Cell and Molecular Biosciences, Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK. Electronic address: harry.gilbert@newcastle.ac.uk.
J Paul KnoxCentre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. Electronic address: j.p.knox@leeds.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/K001949/1Biotechnology and Biological Sciences Research Council BB/K020358/1Wellcome Trust 097907Wellcome Trust WT097907AIA
6 · The paper itself

Abstract

Type A non-catalytic carbohydrate-binding modules (CBMs), exemplified by CtCBM3acipA, are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β-1,4-glucan containing α-1,6-xylose side chains. CtCBM3acipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3acipA. A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM-polysaccharide interactions is discussed.

Indexed as

Acetivibrio thermocellusBacterial ProteinsBinding SitesCelluloseCell WallGlucansMembrane ProteinsProtein BindingSolubilityXylansBacterial ProteinsCelluloseCipA protein, ClostridiumGlucansMembrane ProteinsXylansxyloglucanCarbohydrate-binding moduleCBM3aCrystalline cellulosePlant cell wallXyloglucan

Identifiers

PMID26193423
PMCPMC5877785

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.