ArticleInternational journal of molecular sciences2015
Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?Parasitology · 2020Pooled it
- Identification of Promising Candidate Genes and Immune Infiltration Patterns in Chronic Schistosomiasis-Associated Liver Injury by WGCNA and Machine Learning.Pathogens (Basel, Switzerland) · 2026Article
- Diagnostic Performance and Discordance of Kato-Katz Method, POC-CCA Test, and PCR in DetectingDiseases (Basel, Switzerland) · 2026Article
- Schistosomiasis in Yemen: Focal Persistence, Possible Resurgence, and Challenges to Elimination.Research and reports in tropical medicine · 2026Review
- Analysis of DNA cox1 barcoding revealed novel haplotype in Schistosoma haematobium isolated from Western Sudan.Scientific reports · 2025Article
- A Recent Advance in the Diagnosis, Treatment, and Vaccine Development for Human Schistosomiasis.Tropical medicine and infectious disease · 2024Review
- Schistosomiasis.Advances in experimental medicine and biology · 2024Review
- Bladder stones in a closed diverticulum caused byWorld journal of clinical cases · 2020Article
- Case Report of Urinary Schistosomiasis in a Returned Traveler in Korea.The Korean journal of parasitology · 2020Article
- Prevalence ofJournal of parasitology research · 2020Article
- High-resolution melting analysis for rapid and sensitiveOncology letters · 2019Article
- Improved detection of DNA Schistosoma haematobium from eggs extracted by bead beating in urine.Parasitology research · 2019Article
- Urogenital schistosomiasis is associated with signatures of microbiome dysbiosis in Nigerian adolescents.Scientific reports · 2019Article
- Diagnostic Value of SjR2 Gene in Colonic Tissue from Schistosoma Japonicum Infected Hosts.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- The Advances in Molecular and New Point-of-Care (POC) Diagnosis of Schistosomiasis Pre- and Post-praziquantel Use: In the Pursuit of More Reliable Approaches for Low Endemic and Non-endemic Areas.Frontiers in immunology · 2019Review
- Detection of four patients who were infected byInfection and drug resistance · 2019Article
- DNA Diagnostics for Schistosomiasis Control.Tropical medicine and infectious disease · 2018Review
- Article
- Clinical diagnostic value of viable Schistosoma japonicum eggs detected in host tissues.BMC infectious diseases · 2017Article
- Article
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12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study describes a real-time PCR approach with high resolution melting-curve (HRM) assay developed for the detection and differentiation of Schistosoma mansoni and S. haematobium in fecal and urine samples collected from rural Yemen. The samples were screened by microscopy and PCR for the Schistosoma species infection. A pair of degenerate primers were designed targeting partial regions in the cytochrome oxidase subunit I (cox1) gene of S. mansoni and S. haematobium using real-time PCR-HRM assay. The overall prevalence of schistosomiasis was 31.8%; 23.8% of the participants were infected with S. haematobium and 9.3% were infected with S. mansoni. With regards to the intensity of infections, 22.1% and 77.9% of S. haematobium infections were of heavy and light intensities, respectively. Likewise, 8.1%, 40.5% and 51.4% of S. mansoni infections were of heavy, moderate and light intensities, respectively. The melting points were distinctive for S. mansoni and S. haematobium, categorized by peaks of 76.49 ± 0.25 °C and 75.43 ± 0.26 °C, respectively. HRM analysis showed high detection capability through the amplification of Schistosoma DNA with as low as 0.0001 ng/µL. Significant negative correlations were reported between the real-time PCR-HRM cycle threshold (Ct) values and microscopic egg counts for both S. mansoni in stool and S. haematobium in urine (p < 0.01). In conclusion, this closed-tube HRM protocol provides a potentially powerful screening molecular tool for the detection of S. mansoni and S. haematobium. It is a simple, rapid, accurate, and cost-effective method. Hence, this method is a good alternative approach to probe-based PCR assays.
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