ArticleScientific reports2015
Formation and reshuffling of disulfide bonds in bovine serum albumin demonstrated using tandem mass spectrometry with collision-induced and electron-transfer dissociation.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 96 citations in OpenAlex.
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- Long-term interleukin-4 release from 3D printable affinity hydrogels promotes M2-like macrophage polarisationBiomaterials science · 2025Article
- Potential Mechanisms of Lactate Dehydrogenase and Bovine Serum Albumin Proteins as Antioxidants: A Mixed Experimental-Computational Study.Biochemistry research international · 2025Article
- 4D Printed Protein-AuNR Nanocomposites with Photothermal Shape Recovery.Advanced functional materials · 2024Article
- Influence of protein nativity on the stability of bovine serum albumin coated microbubbles.iScience · 2024Article
- Structure-Activity Relationship of the Dimeric and Oligomeric Forms of a Cytotoxic Biotherapeutic Based on Diphtheria Toxin.Biomolecules · 2022Article
- Isolation and Characterization of Chicken Serum Albumin (Hen Egg Alpha-Livetin, Gal d 5).Foods (Basel, Switzerland) · 2022Article
- Improving Tirapazamine (TPZ) to Target and Eradicate Hypoxia Tumors by Gold Nanoparticle Carriers.Pharmaceutics · 2022Article
- Basophil Activation to Gluten and Non-Gluten Proteins in Wheat-Dependent Exercise-Induced Anaphylaxis.Frontiers in allergy · 2022Article
- Self-Healing Behavior of Polymer/Protein Hybrid Hydrogels.Polymers · 2021Article
- Serum Albumin Redox States: More Than Oxidative Stress Biomarker.Antioxidants (Basel, Switzerland) · 2021Review
- Reactions of Medicinal Gold(III) Compounds With Proteins and Peptides Explored by Electrospray Ionization Mass Spectrometry and Complementary Biophysical Methods.Frontiers in chemistry · 2020Article
- Comprehensive identification of protein disulfide bonds with pepsin/trypsin digestion, Orbitrap HCD and Spectrum Identification Machine.Journal of proteomics · 2019Article
- Article
- Visible-Light-Activated Quinolone Carbon-Monoxide-Releasing Molecule: Prodrug and Albumin-Assisted Delivery Enables Anticancer and Potent Anti-Inflammatory Effects.Journal of the American Chemical Society · 2018Article
- A General Aqueous Silanization Protocol to Introduce Vinyl, Mercapto or Azido Functionalities onto Cellulose Fibers and Nanocelluloses.Molecules (Basel, Switzerland) · 2018Article
- From Protein Features to Sensing Surfaces.Sensors (Basel, Switzerland) · 2018Review
- Iodine-Containing Mass-Defect-Tuned Dendrimers for Use as Internal Mass Spectrometry Calibrants.Journal of the American Society for Mass Spectrometry · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermolysin hydrolyzates of freshly isolated, extensively stored (6 years, 6 °C, dry) and heated (60 min, 90 °C, in excess water) bovine serum albumin (BSA) samples were analyzed with liquid chromatography (LC) electrospray ionization (ESI) tandem mass spectrometry (MS/MS) using alternating electron-transfer dissociation (ETD) and collision-induced dissociation (CID). The positions of disulfide bonds and free thiol groups in the different samples were compared to those deduced from the crystal structure of native BSA. Results revealed non-enzymatic posttranslational modifications of cysteine during isolation, extensive dry storage, and heating. Heat-induced extractability loss of BSA was linked to the impact of protein unfolding on the involvement of specific cysteine residues in intermolecular and intramolecular thiol-disulfide interchange and thiol oxidation reactions. The here developed approach holds promise for exploring disulfide bond formation and reshuffling in various proteins under conditions relevant for chemical, biochemical, pharmaceutical and food processing.
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