Evidence map›Paper›PMID 26192439›Full record

ArticleNature cell biology2015

Generation of stomach tissue from mouse embryonic stem cells.

Taka-aki K Noguchi, Naoto Ninomiya, Mari Sekine, Shinji Komazaki, Pi-Chao Wang, Makoto Asashima, Akira Kurisaki

Abstract read
PubMed Publisher
In one paragraph

Article in Nature cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 104 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Engineered organoids for biomedical applications.Advanced drug delivery reviews · 2023
    Review
  9. Review
  10. Review
  11. Review
  12. Article
  13. Tissue Engineering for Gastrointestinal and Genitourinary Tracts.International journal of molecular sciences · 2022
    Review
  14. Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Gastric Organoids: Progress and Remaining Challenges.Cellular and molecular gastroenterology and hepatology · 2022
    Review
  20. Biobanking of human gut organoids for translational research.Experimental & molecular medicine · 2021
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Taka-aki K NoguchiGraduate School of Life and Environmental Sciences, The University of Tsukuba, Ibaraki 305-8577, Japan.
Naoto NinomiyaResearch Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki 305-8562, Japan.
Mari SekineGraduate School of Life and Environmental Sciences, The University of Tsukuba, Ibaraki 305-8577, Japan.
Shinji KomazakiDepartment of Anatomy, Saitama Medical University, Saitama 350-0495, Japan.
Pi-Chao WangGraduate School of Life and Environmental Sciences, The University of Tsukuba, Ibaraki 305-8577, Japan.
Makoto Asashima1] Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki 305-8562, Japan [2] Life Science Center of Tsukuba Advanced Research Alliance, The University of Tsukuba, Ibaraki 305-8577, Japan.
Akira Kurisaki1] Graduate School of Life and Environmental Sciences, The University of Tsukuba, Ibaraki 305-8577, Japan [2] Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki 305-8562, Japan.
University of Tsukuba · JPNational Institute of Advanced Industrial Science and Technology · JPSaitama Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Successful pluripotent stem cell differentiation methods have been developed for several endoderm-derived cells, including hepatocytes, β-cells and intestinal cells. However, stomach lineage commitment from pluripotent stem cells has remained a challenge, and only antrum specification has been demonstrated. We established a method for stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1, an essential gene for in vivo stomach specification from gut endoderm. Barx1-inducing culture conditions generated stomach primordium-like spheroids, which differentiated into mature stomach tissue cells in both the corpus and antrum by three-dimensional culture. This embryonic stem cell-derived stomach tissue (e-ST) shared a similar gene expression profile with adult stomach, and secreted pepsinogen as well as gastric acid. Furthermore, TGFA overexpression in e-ST caused hypertrophic mucus and gastric anacidity, which mimicked Ménétrier disease in vitro. Thus, in vitro stomach tissue derived from pluripotent stem cells mimics in vivo development and can be used for stomach disease models.

Indexed as

Cell DifferentiationAnimalsCell LineageEmbryonic Stem CellsGastric AcidGastric MucosaGastritis, HypertrophicGene Expression ProfilingGene Expression Regulation, DevelopmentalHedgehog ProteinsHistamineHomeodomain ProteinsMiceMice, Inbred ICROrganogenesisPepsinogen ABarx1 protein, mouseHedgehog ProteinsHistamineHomeodomain ProteinsPepsinogen ATGFA protein, humanTranscription FactorsTransforming Growth Factor alpha

Identifiers

PMID26192439
OpenAlexW1953113928

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.