ArticlePloS one2015
Improved Gene Targeting through Cell Cycle Synchronization.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 77 citations in OpenAlex.
- A CRISPR-Cas9 Toolkit Enabling Tunable Integration and Transient Homologous Recombination Enhancement in Yarrowia lipolytica.Biotechnology and bioengineering · 2026Article
- Engineering de novo formatotrophy in the non-model yeastNature synthesis · 2026Article
- Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future Innovations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- The rise and future of CRISPR-based approaches for high-throughput genomics.FEMS microbiology reviews · 2024Review
- Improving homology-directed repair by small molecule agents for genetic engineering in unconventional yeast?-Learning from the engineering of mammalian systems.Microbial biotechnology · 2024Review
- The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing.Synthetic and systems biotechnology · 2023Review
- Timing of Chromosome DNA Integration throughout the Yeast Cell Cycle.Biomolecules · 2023Article
- Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeast Candida intermedia.FEMS yeast research · 2023Article
- Modular co-culture engineering of Yarrowia lipolytica for amorphadiene biosynthesis.Microbial cell factories · 2022Article
- A New Expression System Based on PsychrotolerantInternational journal of molecular sciences · 2022Article
- Article
- Improving Homology-Directed Repair in Genome Editing Experiments by Influencing the Cell Cycle.International journal of molecular sciences · 2022Review
- Advance trends in targeting homology-directed repair for accurate gene editing: An inclusive review of small molecules and modified CRISPR-Cas9 systems.BioImpacts : BI · 2022Review
- Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production.Applied microbiology and biotechnology · 2021Review
- Increasing lipid yield in Yarrowia lipolytica through phosphoketolase and phosphotransacetylase expression in a phosphofructokinase deletion strain.Biotechnology for biofuels · 2021Article
- Transcriptome Response of the Tropical Marine Yeast Yarrowia lipolytica on Exposure to Uranium.Current microbiology · 2021Article
- A CRISPR/Cas9-Mediated, Homology-Independent Tool Developed for Targeted Genome Integration in Yarrowia lipolytica.Applied and environmental microbiology · 2021Article
- Variation amongMicroorganisms · 2021Article
- Production of 10-methyl branched fatty acids in yeast.Biotechnology for biofuels · 2021Article
- Targeted Integration Through Transformation of Hydroxyurea-Arrested Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during transformation. Using hydroxyurea-mediated cell cycle arrest, we obtained improved gene targeting rates in Yarrowia lipolytica, Arxula adeninivorans, Saccharomyces cerevisiae, Kluyveromyces lactis and Pichia pastoris demonstrating the broad applicability of the method. Hydroxyurea treatment enriches for S-phase cells that are active in homologous recombination and enables previously unattainable genomic modifications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.