ArticleJournal of natural products2015
Dereplicating and Spatial Mapping of Secondary Metabolites from Fungal Cultures in Situ.
Article in Journal of natural products, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Paraisariamides: Cycloheptapeptide Toxins from Entomopathogenic Fungi (Journal of natural products · 2026Article
- Scaling up the production of fungal perylenequinones and investigating their biosynthesis through stable isotope labeling.Microbial cell factories · 2025Article
- Bioactivity-driven fungal metabologenomics identifies antiproliferative stemphone analogs and their biosynthetic gene cluster.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Dereplication of Fungal Metabolites by NMR-Based Compound Networking Using MADByTE.Journal of natural products · 2022Article
- Discovery of Anticancer Agents of Diverse Natural Origin.Journal of natural products · 2022Article
- SubTap, a Versatile 3D Printed Platform for Eavesdropping on Extracellular Interactions.mSystems · 2021Article
- Fungal Guttation, a Source of Bioactive Compounds, and Its Ecological Role-A Review.Biomolecules · 2021Review
- Article
- Cytotoxic Naphthoquinone Analogues, Including Heterodimers, and Their Structure Elucidation Using LR-HSQMBC NMR Experiments.Journal of natural products · 2021Article
- Drug Leads from Endophytic Fungi: Lessons Learned via Scaled Production.Planta medica · 2020Article
- Mass Spectrometry: A Rosetta Stone to Learn How Fungi Interact and Talk.Life (Basel, Switzerland) · 2020Review
- Orthogonal Method for Double-Bond Placement via Ozone-Induced Dissociation Mass Spectrometry (OzID-MS).Journal of natural products · 2019Article
- Engineering Fluorine into Verticillins (Epipolythiodioxopiperazine Alkaloids) via Precursor-Directed Biosynthesis.Journal of natural products · 2019Article
- Rapid Characterization of Chemical Components in Edible MushroomMolecules (Basel, Switzerland) · 2019Article
- Droplet probe: coupling chromatography to the in situ evaluation of the chemistry of nature.Natural product reports · 2019Review
- Mapping the Fungal Battlefield: UsingFrontiers in microbiology · 2019Article
- Non-Destructive Chemical Analysis of aPhytochemistry letters · 2018Article
- Media studies to enhance the production of verticillins facilitated by in situ chemical analysis.Journal of industrial microbiology & biotechnology · 2018Article
- Calothrixamides A and B from the Cultured Cyanobacterium Calothrix sp. UIC 10520.Journal of natural products · 2018Article
- Improving the discovery of secondary metabolite natural products using ion mobility-mass spectrometry.Current opinion in chemical biology · 2018Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Ambient ionization mass spectrometry techniques have recently become prevalent in natural product research due to their ability to examine secondary metabolites in situ. These techniques retain invaluable spatial and temporal details that are lost through traditional extraction processes. However, most ambient ionization techniques do not collect mutually supportive data, such as chromatographic retention times and/or UV/vis spectra, and this can limit the ability to identify certain metabolites, such as differentiating isomers. To overcome this, the droplet-liquid microjunction-surface sampling probe (droplet-LMJ-SSP) was coupled with UPLC-PDA-HRMS-MS/MS, thus providing separation, retention times, MS data, and UV/vis data used in traditional dereplication protocols. By capturing these mutually supportive data, the identity of secondary metabolites can be confidently and rapidly assigned in situ. Using the droplet-LMJ-SSP, a protocol was constructed to analyze the secondary metabolite profile of fungal cultures without any sample preparation. The results demonstrate that fungal cultures can be dereplicated from the Petri dish, thus identifying secondary metabolites, including isomers, and confirming them against reference standards. Furthermore, heat maps, similar to mass spectrometry imaging, can be used to ascertain the location and relative concentration of secondary metabolites directly on the surface and/or surroundings of a fungal culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.