Evidence map›Paper›PMID 26192107›Full record

ArticleMolecular endocrinology (Baltimore, Md.)2015

Ubc9 Impairs Activation of the Brown Fat Energy Metabolism Program in Human White Adipocytes.

Sean M Hartig, David A Bader, Kathleen V Abadie, Massoud Motamed, Mark P Hamilton, Weiwen Long, Brian York, Michaela Mueller, Martin Wagner, Michael Trauner and 5 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular endocrinology (Baltimore, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 15 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. American journal of physiology. Endocrinology and metabolism · 2020
    Article
  7. A Provocative Molecular Link between Mammographic Density and BRCA1-loss associated TNBC.International journal of human genetics and genetic disorders · 2019
    Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 2 countries.

Sean M HartigDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
David A BaderDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Kathleen V AbadieDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Massoud MotamedDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Mark P HamiltonDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Weiwen LongDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Brian YorkDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Michaela MuellerDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Martin WagnerDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Michael TraunerDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Lawrence ChanDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Mandeep BajajDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
David D MooreDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Michael A ManciniDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Sean E McGuireDepartment of Molecular and Cellular Biology (S.M.H., D.A.B., K.V.A., M.Mo., M.P.H., W.L., B.Y., L.C., D.D.M., M.A.M., S.E.M.), Baylor College of Medicine, Houston, Texas 77030; Department of Biochemistry and Molecular Biology (W.L.), Wright State University Boonshoft School of Medicine, Dayton, Ohio 45435; Hans Popper Laboratory of Molecular Hepatology (M.Mu., M.T.), Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Vienna, Austria; Laboratory of Experimental Hepatology (M.W.), Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria; Diabetes and Endocrinology Research Center (L.C., M.B.), Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Baylor College of Medicine, and the Baylor St Luke's Medical Center, Houston, Texas 77030; and Division of Radiation Oncology (S.E.M.), The University of Texas MD Anderson Cancer Center, Houston, Texas 77030.
Baylor College of Medicine · USMedical University of Vienna · ATMedical University of Graz · ATThe University of Texas MD Anderson Cancer Center · USWright State University · US

Funding

Tumor BiologyP30CA125123 · NCI · BAYLOR COLLEGE OF MEDICINE · PI DAVID R ROWLEY · 2007 to 2026
$73.9M
Tissue Analysis & Molecular Imaging CoreP30DK056338 · NIDDK · BAYLOR COLLEGE OF MEDICINE · PI Geoffrey A Preidis · 2001 to 2026
$28.3M
TRANSGENIC MOUSE MODELS TO STUDY OVARIAN FUNCTIONU54HD007495 · NICHD · BAYLOR COLLEGE OF MEDICINE · PI RICHARDS, JOANNE STEWART · 1998 to 2013
$19.9M
Diabetes Endocrinology Research CenterP30DK079638 · NIDDK · BAYLOR COLLEGE OF MEDICINE · PI CHAN, LAWRENCE · 2008 to 2012
$6.4M
STRUCTURE/FUNCTION OF GENETIC ENGINEERED CALMODULINSR01GM033976 · NIGMS · DUKE UNIVERSITY · PI MEANS, ANTHONY R · 1985 to 2015
$5.4M
Epigenetic Programmers Targeted During Developmental ReprogrammingR01ES023206 · NIEHS · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI BEDFORD, MARK T., MANCINI, MICHAEL A. · 2013 to 2016
$2.3M
Developmental Reprogramming of Prostate Carcinogenesis by BPARC2ES018789 · NIEHS · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI WALKER, CHERYL L. · 2009 to 2010
$1.9M
A novel axis regulates adipocyte plasticityK01DK096093 · NIDDK · BAYLOR COLLEGE OF MEDICINE · PI HARTIG, SEAN · 2013 to 2015
$444k
NCI NIH HHS P30 CA125123NICHD NIH HHS U54 HD-007495NICHD NIH HHS U54 HD007495NIDDK NIH HHS K01 DK096093NIDDK NIH HHS K01DK096093NIDDK NIH HHS P30 DK056338NIDDK NIH HHS P30 DK079638NIDDK NIH HHS P30-DK079638NIDDK NIH HHS P30 DK-56338NIEHS NIH HHS 1R01ES023206NIEHS NIH HHS 1RC2ES018789NIEHS NIH HHS R01 ES023206NIEHS NIH HHS RC2 ES018789NIGMS NIH HHS R01 GM033976NIGMS NIH HHS R01GM033976
6 · The paper itself

Abstract

Insulin resistance and type 2 diabetes mellitus (T2DM) result from an inability to efficiently store and catabolize surplus energy in adipose tissue. Subcutaneous adipocytes protect against insulin resistance and T2DM by coupling differentiation with the induction of brown fat gene programs for efficient energy metabolism. Mechanisms that disrupt these programs in adipocytes are currently poorly defined, but represent therapeutic targets for the treatment of T2DM. To gain insight into these mechanisms, we performed a high-throughput microscopy screen that identified ubiquitin carrier protein 9 (Ubc9) as a negative regulator of energy storage in human sc adipocytes. Ubc9 depletion enhanced energy storage and induced the brown fat gene program in human sc adipocytes. Induction of adipocyte differentiation resulted in decreased Ubc9 expression commensurate with increased brown fat gene expression. Thiazolidinedione treatment reduced the interaction between Ubc9 and peroxisome proliferator-activated receptor (PPAR)γ, suggesting a mechanism by which Ubc9 represses PPARγ activity. In support of this hypothesis, Ubc9 overexpression remodeled energy metabolism in human sc adipocytes by selectively inhibiting brown adipocyte-specific function. Further, Ubc9 overexpression decreased uncoupling protein 1 expression by disrupting PPARγ binding at a critical uncoupling protein 1 enhancer region. Last, Ubc9 is significantly elevated in sc adipose tissue isolated from mouse models of insulin resistance as well as diabetic and insulin-resistant humans. Taken together, our findings demonstrate a critical role for Ubc9 in the regulation of sc adipocyte energy homeostasis.

Indexed as

Adipocytes, WhiteAdipose Tissue, BrownAnimalsDiabetes Mellitus, Type 2Disease Models, AnimalEnergy MetabolismHigh-Throughput Screening AssaysHumansHypoglycemic AgentsInsulin ResistanceMaleMiceMice, Inbred C57BLPPAR gammaRNA InterferenceRNA, Small InterferingHypoglycemic AgentsPPAR gammaRNA, Small InterferingRosiglitazoneThiazolidinedionesUbiquitin-Conjugating EnzymesUbiquitin-Conjugating Enzyme UBC9

Identifiers

PMID26192107
PMCPMC4552434
OpenAlexW2201093393

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.