ArticlePloS one2015
Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 107 citations in OpenAlex.
- Preparation and characterization of decellularized bovine bone as a bioscaffold for bone tissue engineering applications.Iranian journal of basic medical sciences · 2026Article
- Bone-Derived dECM Hydrogels Support Tunable Microenvironments for In Vitro Osteogenic Differentiation.Advanced healthcare materials · 2026Article
- Application of decellularization methods for scaffold production: advantages, disadvantages, biosafety and modifications.Frontiers in bioengineering and biotechnology · 2025Review
- Article
- Biological parameters for quality evaluation of allografts from the Brazilian National Institute of Traumatology and Orthopedics tissue bank.Cell and tissue banking · 2024Article
- An overview of the production of tissue extracellular matrix and decellularization process.Cell and tissue banking · 2024Review
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- A novel Chilean salmon fish backbone-based nanoHydroxyApatite functional biomaterial for potential use in bone tissue engineering.Frontiers in medicine · 2024Article
- Article
- Evaluation of decellularization process for developing osteogenic bovine cancellous bone scaffolds in-vitro.PloS one · 2023Article
- In vitro evaluation of crosslinked bovine pericardium as potential scaffold for the oral cavity.Bio-medical materials and engineering · 2023Article
- Regenerative Efficacy of Supercritical Carbon Dioxide-Derived Bone Graft Putty in Rabbit Bone Defect Model.Biomedicines · 2022Article
- The comprehensive on-demand 3D bio-printing for composite reconstruction of mandibular defects.Maxillofacial plastic and reconstructive surgery · 2022Review
- In Vitro Model for the Evaluation of Innovative Transcatheter Debridement Device (TDD): Pericardium-Based Scaffold and Stem Cells to Reproduce Calcificated Valves.Biomedicines · 2022Article
- Assessment of the efficiency of different chemical treatments and ultrasonic cleaning for defatting of cancellous bone samples.Cell and tissue banking · 2022Article
- The effect of Scrophularia striata on cell attachment and biocompatibility of decellularized bovine pericardia.Cell and tissue banking · 2022Article
- Decellularized xenogenic bone graft for repair of segmental bone defect in rabbits.Iranian journal of veterinary research · 2022Article
- Evaluation of cell adhesion and osteoconductivity in bone substitutes modified by polydopamine.Frontiers in bioengineering and biotechnology · 2022Review
- Cutting Edge Endogenous Promoting and Exogenous Driven Strategies for Bone Regeneration.International journal of molecular sciences · 2021Review
- Bioresorbable Magnesium-Based Alloys as Novel Biomaterials in Oral Bone Regeneration: General Review and Clinical Perspectives.Journal of clinical medicine · 2021Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The combination of bone grafting materials with guided bone regeneration (GBR) membranes seems to provide promising results to restore bone defects in dental clinical practice. In the first part of this work, a novel protocol for decellularization and delipidation of bovine bone, based on multiple steps of thermal shock, washes with detergent and dehydration with alcohol, is described. This protocol is more effective in removal of cellular materials, and shows superior biocompatibility compared to other three methods tested in this study. Furthermore, histological and morphological analyses confirm the maintenance of an intact bone extracellular matrix (ECM). In vitro and in vivo experiments evidence osteoinductive and osteoconductive properties of the produced scaffold, respectively. In the second part of this study, two methods of bovine pericardium decellularization are compared. The osmotic shock-based protocol gives better results in terms of removal of cell components, biocompatibility, maintenance of native ECM structure, and host tissue reaction, in respect to the freeze/thaw method. Overall, the results of this study demonstrate the characterization of a novel protocol for the decellularization of bovine bone to be used as bone graft, and the acquisition of a method to produce a pericardium membrane suitable for GBR applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.