Evidence map›Paper›PMID 26191773›Full record

ArticleJournal of molecular biology2015

A Regulated, Ubiquitin-Independent Degron in IκBα.

Karen T Fortmann, Russell D Lewis, Kim A Ngo, Riku Fagerlund, Alexander Hoffmann

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Functional importance of stripping in NFκB signaling revealed by a stripping-impaired IκBα mutant.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  11. Article
  12. Signaling via the NFκB system.Wiley interdisciplinary reviews. Systems biology and medicine · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Karen T FortmannDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
Russell D LewisDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
Kim A NgoDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA; Institute for Quantitative and Computational Biosciences, Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Riku FagerlundDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
Alexander HoffmannDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA; Institute for Quantitative and Computational Biosciences, Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
University of California San Diego · USUniversity of California, Los Angeles · US

Funding

STRUCTURE AND STABILITY OF IKB/NF-KB COMPLEXESP01GM071862 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KOMIVES, ELIZABETH A. · 2006 to 2016
$14.9M
IKK:Biophysical basis of dynamic regulationR01CA141722 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GHOSH, GOURISANKAR, HOFFMANN, ALEXANDER · 2009 to 2013
$2.7M
Regulation of signaling via the lkB-NF-kB interactionR01GM071573 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HOFFMANN, ALEXANDER · 2005 to 2013
$2.2M
NCI NIH HHS R01 CA141722NIGMS NIH HHS P01 GM071862NIGMS NIH HHS R01 GM071573
6 · The paper itself

Abstract

Whereas ubiquitin-dependent degrons have been characterized in some detail, how proteins may be targeted to ubiquitin-independent proteasomal degradation remains unclear. Here we show that IκBα contains an ubiquitin-independent degron whose activity is portable to heterologous proteins such as the globular protein GFP (green fluorescent protein) via a proteasome-dependent, ubiquitin-independent, non-lysosomal pathway. The ubiquitin-independent degradation signal resides in an 11-amino-acid sequence, which is not only sufficient but also required for IκBα's short half-life. Finally, we show that this degron's activity is regulated by the interaction with NFκB, which controls its solvent exposure, and we demonstrate that this regulation of the degron's activity is critical for IκBα's signaling functions.

Indexed as

3T3 CellsAmino Acid SequenceAnimalsAnkyrin RepeatCell LineChloroquineCycloheximideDNA-Binding ProteinsGreen Fluorescent ProteinsHEK293 CellsHumansI-kappa B ProteinsLysosomesMacrolidesMiceNF-KappaB Inhibitor alphabafilomycin AChloroquineCycloheximideDNA-Binding ProteinsGreen Fluorescent ProteinsI-kappa B ProteinsMacrolidesNF-KappaB Inhibitor alphaNFKBIA protein, humanNfkbia protein, mouseRela protein, mouseTranscription Factor RelAUbiquitindegronIκBαNFκBprotein half-life controlubiquitin-independent degradation

Identifiers

PMID26191773
PMCPMC4685248
OpenAlexW1771156064

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.