ArticleApplications in plant sciences2015
A complementation assay for in vivo protein structure/function analysis in Physcomitrella patens (Funariaceae).
Article in Applications in plant sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Diversification of Cellulose Synthase (CESA) Genes in Mosses Suggests Both Ancient and Recent Gene duplications.Journal of molecular evolution · 2026Article
- The Sulfated PSY Peptide Negatively Regulates Receptor Kinase Activity to Promote Growth.bioRxiv : the preprint server for biology · 2026Article
- Cellulose synthase-like D movement in the plasma membrane requires enzymatic activity.The Journal of cell biology · 2023Article
- Cellulose synthesis complexes are homo-oligomeric and hetero-oligomeric in Physcomitrium patens.Plant physiology · 2022Article
- Phenotypic effects of changes in the FTVTxK region of anPlant direct · 2021Article
- Convergent evolution of hetero-oligomeric cellulose synthesis complexes in mosses and seed plants.The Plant journal : for cell and molecular biology · 2019Article
- Functional Characterization of a Glycosyltransferase from the MossThe Plant cell · 2018Article
- Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens.Scientific reports · 2018Article
- Functional and Structural Diversity of Acyl-coA Binding Proteins in Oil Crops.Frontiers in genetics · 2018Review
- Functional Specialization of Cellulose Synthase Isoforms in a Moss Shows Parallels with Seed Plants.Plant physiology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
premise of the studyA method for rapid in vivo functional analysis of engineered proteins was developed using Physcomitrella patens. METHODS AND
resultsA complementation assay was designed for testing structure/function relationships in cellulose synthase (CESA) proteins. The components of the assay include (1) construction of test vectors that drive expression of epitope-tagged PpCESA5 carrying engineered mutations, (2) transformation of a ppcesa5 knockout line that fails to produce gametophores with test and control vectors, (3) scoring the stable transformants for gametophore production, (4) statistical analysis comparing complementation rates for test vectors to positive and negative control vectors, and (5) analysis of transgenic protein expression by Western blotting. The assay distinguished mutations that generate fully functional, nonfunctional, and partially functional proteins.
conclusionsCompared with existing methods for in vivo testing of protein function, this complementation assay provides a rapid method for investigating protein structure/function relationships in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.