Evidence map›Paper›PMID 26190923›Full record

ArticleMycobiology2015

Optimization of Protein Extraction for Lichen Thalli.

Anna S Kondratiuk, Oleksiy M Savchuk, Jae-Seoun Hur

Open access · hybridAbstract read
In one paragraph

Article in Mycobiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.5field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Anna S KondratiukInstitute of Biology, Scientific Educational Center, Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine. ; Korean Lichen Research Institute, Sunchon National University, Suncheon 540-950, Korea.
Oleksiy M SavchukInstitute of Biology, Scientific Educational Center, Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine.
Jae-Seoun HurKorean Lichen Research Institute, Sunchon National University, Suncheon 540-950, Korea.
Sunchon National University · KRTaras Shevchenko National University of Kyiv · UA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study.

Indexed as

ElectrophoresisOptimization methodPhenolsProteinsQuinones

Identifiers

PMID26190923
PMCPMC4505004
OpenAlexW927165779

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.