Evidence map›Paper›PMID 26190543›Full record

ArticleNeurogastroenterology and motility2015

Endoscopic delivery of enteric neural stem cells to treat Hirschsprung disease.

L S Cheng, R Hotta, H K Graham, N Nagy, A M Goldstein, J Belkind-Gerson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurogastroenterology and motility, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
5.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 37 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

L S ChengDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
R HottaDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
H K GrahamDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
N NagyDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
A M GoldsteinDepartment of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
J Belkind-GersonPediatric Neurogastroenterology Program, MassGeneral Hospital for Children, Boston, MA, USA.
MaineGeneral Medical Center · USMassachusetts General Hospital · USHarvard University · USSemmelweis University · HU

Funding

Gdnf and endothelin-3 regulate colorectal enteric nervous system developmentR01DK080914 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI GOLDSTEIN, ALLAN M · 2009 to 2013
$2.0M
Developmentally Informed Approach to Cell-based Therapy for Enteric NeuropathiesR01DK103785 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI GOLDSTEIN, ALLAN M · 2015 to 2019
$1.9M
NIDDK NIH HHS DK080914NIDDK NIH HHS R01 DK080914NIDDK NIH HHS R01 DK103785
6 · The paper itself

Abstract

backgroundTransplantation of enteric neural stem cells (ENSC) holds promise as a potential therapy for enteric neuropathies, including Hirschsprung disease. Delivery of transplantable cells via laparotomy has been described, but we propose a novel, minimally invasive endoscopic method of cell delivery.

methodsEnteric neural stem cells for transplantation were cultured from dissociated gut of postnatal donor mice. Twelve recipient mice, including Ednrb(-/-) mice with distal colonic aganglionosis, underwent colonoscopic injection of ENSC under direct vision using a 30-gauge Hamilton needle passed through a rigid cystoureteroscope. Cell engraftment, survival, and neuroglial differentiation were studied 1-4 weeks after the procedure. KEY

resultsAll recipient mice tolerated the procedure without complications and survived to sacrifice. Transplanted cells were found within the colonic wall in 9 of 12 recipient mice with differentiation into enteric neurons and glia. CONCLUSIONS & INFERENCES: Endoscopic injection of ENSC is a safe and reliable method for cell delivery, and can be used to deliver a large number of cells to a specific area of disease. This minimally invasive endoscopic approach may prove beneficial to future human applications of cell therapy for neurointestinal disease.

Indexed as

AnimalsColonoscopyDisease Models, AnimalEnteric Nervous SystemHirschsprung DiseaseMiceMice, 129 StrainMice, Inbred C57BLMice, TransgenicNeural Stem CellsReceptor, Endothelin BEDNRB protein, mouseReceptor, Endothelin Bcell therapyendoscopyenteric neural stem cellsenteric neuropathiesHirschsprung disease

Identifiers

PMID26190543
PMCPMC4600089
OpenAlexW2159305532

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.